The XadA Trimeric Autotransporter Adhesins in Xylella fastidiosa Differentially Contribute to Cell Aggregation, Biofilm Formation, Insect Transmission and Virulence to Plants

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Feitosa-Junior, Oseias R. ; Souza, Ana Paula S. ; Zaini, Paulo A. ; Baccari, Clelia ; Ionescu, Michael ; Pierry, Paulo M. ; Uceda-Campos, Guillermo ; Labroussaa, Fabien ; Almeida, Rodrigo P. P. ; Lindow, Steven E. ; da Silva, Aline M.

• Tipo de documento: Artigo Científico
• Fonte: MOLECULAR PLANT-MICROBE INTERACTIONS; v. 35, n. 9, p. 10-pg., 2022-09-01.

Resumo

Surface adhesion strategies are widely employed by bacterial pathogens during establishment and systemic spread in their host. A variety of cell-surface appendages such as pili, fimbriae, and afimbrial adhesins are involved in these processes. The phytopathogen Xylella fastidiosa employs several of these structures for efficient colonization of its insect and plant hosts. Among the adhesins encoded in the X. fastidiosa genome, three afimbrial adhesins, XadA1, Hsf/XadA2, and XadA3, are predicted to be trimeric autotransporters with aC-terminalYadA-anchor membrane domain. We analyzed the individual contributions of XadA1, XadA2, andXadA3 to various cellular behaviors both in vitro and in vivo. Using isogenic X. fastidiosa mutants, we found that cell-cell aggregation and biofilm formation were severely impaired in the absence of XadA3. No significant reduction of cell-surface attachment was found with any mutant under flow conditions. Acquisition by insect vectors and transmission to grapevines were reduced in the XadA3 deletion mutant. While the XadA3 mutant was hypervirulent in grapevines, XadA1 or XadA2 deletion mutants conferred lower disease severity than the wild-type strain. This insight of the importance of these adhesive proteins and their individual contributions to different aspects of X. fastidiosa biology should guide new approaches to reduce pathogen transmission and disease development. (AU)

• Processo FAPESP: 11/09409-3 - Tecnologia microfluídica para estudo dos mecanismos de adesão de bactérias fitopatogênicas
• Beneficiário: Paulo Adriano Zaini
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado
• Processo FAPESP: 11/24091-0 - Análise do transcriptoma de cepas de Xylella Fastidiosa isoladas de diferentes hospedeiros e busca por RNAs regulatórios
• Beneficiário: Paulo Marques Pierry
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 14/03617-1 - Caracterização bioquímica e funcional da adesina XadA3 de Xylella fastidiosa
• Beneficiário: Ana Paula Silva de Souza
• Modalidade de apoio: Bolsas no Brasil - Doutorado Direto
• Processo FAPESP: 10/16409-7 - Aspectos moleculares da interação de Xylella fastidiosa com seus hospedeiros vegetais
• Beneficiário: Aline Maria da Silva
• Modalidade de apoio: Bolsas no Exterior - Pesquisa
• Processo FAPESP: 08/11703-4 - Identificação de genes potencialmente associados adaptação, evolução e virulência de Xylella fastidiosa
• Beneficiário: Aline Maria da Silva
• Modalidade de apoio: Auxílio à Pesquisa - Regular