DNase enhances photodynamic therapy against fluconazole-resistant Candida albicans biofilms

Autor(es)

Abreu-Pereira, Cesar Augusto ; Klein, Marlise Inez ; Lobo, Carmelia Isabel Vitorino ; Gorayb Pereira, Ana Luiza ; Jordao, Claudia Carolina ; Pavarina, Ana Claudia

• Tipo de documento: Artigo Científico
• Fonte: ORAL DISEASES; v. N/A, p. 13-pg., 2022-02-19.

Resumo

Objective This study evaluated the effectiveness of DNase I combined with antimicrobial photodynamic therapy, mediated by Photodithazine(R) and light-emitting diode light, against biofilms formed by a fluconazole-resistant Candida albicans strain (ATCC 96901) and two clinical isolates (R14 and R70). Materials and Methods Biofilms were grown for 48 h and exposed to DNase for 5 min, followed by application of a photosensitizer (P) and light (L), either singly or combined (P+L+, P-L+, P+L-, P-L-, P-L-DNase, P+L+DNase, P+L-DNase, and P-L+DNase; n = 12). Biofilm analysis included quantification of extracellular matrix components (water-soluble and insoluble polysaccharides, proteins and extracellular DNA), and biomass (total and insoluble), as well as the enumeration of colony-forming units. The data were analyzed using three-way analysis of variance with Bonferroni's post hoc test. Results The DNase treatment combined with aPDT showed a reduction of 1.92, 1.65, and 1.29 log(10) of cell viability compared with untreated controls for ATCC 96901, R14, and R70 strains, respectively. It also reduced extracellular matrix contents of water-soluble polysaccharides (36.3%) and extracellular DNA (72.3%), as well as insoluble biomass content (43.3%). Conclusion The three strains showed similar behavior when treated with DNase, and the extracellular matrix components were affected, improving the effectiveness of antimicrobial photodynamic therapy. (AU)

• Processo FAPESP: 19/11602-8 - Avaliação da eficácia da DNase associada à terapia fotodinâmica na inativação de biofilmes de isolados clínicos de Candida albicans resistente a fluconazol
• Beneficiário: César Augusto Abreu Pereira
• Modalidade de apoio: Bolsas no Brasil - Mestrado
• Processo FAPESP: 14/50857-8 - INCT 2014 - de Óptica Básica e Aplicada às Ciências da Vida
• Beneficiário: Vanderlei Salvador Bagnato
• Modalidade de apoio: Auxílio à Pesquisa - Temático
• Processo FAPESP: 13/07276-1 - CEPOF - Centro de Pesquisa em Óptica e Fotônica
• Beneficiário: Vanderlei Salvador Bagnato
• Modalidade de apoio: Auxílio à Pesquisa - Centros de Pesquisa, Inovação e Difusão - CEPIDs