Monitoring Stimulated Darkening from UV-C Light on Different Bean Genotypes by NMR Spectroscopy

Autor(es)

Salvador, Marilia Vilela ; Crizostomo Kock, Flavio Vinicius ; Santos, Isabella Laporte ; Carvalho Paulino, Jean Fausto ; de Almeida, Caleo Panhoca ; dos Santos Garcia, Rodrigo Henrique ; Benchimol-Reis, Luciana Lasry ; Colnago, Luiz Alberto ; Ferreira, Antonio Gilberto

• Tipo de documento: Artigo Científico
• Fonte: Molecules; v. 27, n. 7, p. 10-pg., 2022-04-01.

Resumo

The use of UV-C cool white light on bean (Phaseolus vulgaris L.) seeds significantly increases the biochemical seed coat post-harvest darkening process, whilst preserving seed germination. The aim of this work consists in monitoring the effect caused by the incidence of UV-C light on different bean genotypes using NMR spectroscopy. The genotype samples named IAC Alvorada; TAA Dama; BRS Estilo and BRS Perola from the Agronomic Institute (IAC; Campinas; SP; Brazil) were evaluated. The following two methodologies were used: a prolonged darkening, in which the grain is placed in a room at a controlled temperature (298 K) and humidity for 90 days, simulating the supermarket shelf; an accelerated darkening, where the grains are exposed to UV-C light (254 nm) for 96 h. The experiments were performed using the following innovative time-domain (TD) NMR approaches: the RK-ROSE pulse sequence; one- and two-dimensional high resolution (HR) NMR experiments (H-1; H-1-H-1 COSY and H-1-C-13 HSQC); chemometrics tools, such as PLS-DA and heat plots. The results suggest that the observed darkening occurs on the tegument after prolonged (90 days) and accelerated (96 h) conditions. In addition, the results indicate that phenylalanine is the relevant metabolite within this context, being able to participate in the chemical reactions accounted for by the darkening processes. Additionally, it is possible to confirm that a UV-C lamp accelerates oxidative enzymatic reactions and that the NMR methods used were a trustworthy approach to monitor and understand the darkening in bean seeds at metabolite level. (AU)

• Processo FAPESP: 21/12694-3 - Aplicações das espectroscopias de RMN e infravermelho na análise não-destrutiva de produtos e processos agroindustriais
• Beneficiário: Luiz Alberto Colnago
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 18/16040-5 - Controle Biorracional de Insetos Pragas
• Beneficiário: Flavio Vinicius Crizostomo Kock
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado
• Processo FAPESP: 18/08582-2 - Estudo de associação ampla do genoma para resistência à Fusarium oxysporum f. SP. phaseoli e para cor de tegumento em feijoeiro comum
• Beneficiário: Marília Vilela Salvador
• Modalidade de apoio: Bolsas no Brasil - Programa Capacitação - Treinamento Técnico
• Processo FAPESP: 17/24711-4 - Estudo de associação ampla do genoma para resistência à Fusarium oxysporum f. sp. phaseoli e para cor de tegumento em feijoeiro comum
• Beneficiário: Luciana Lasry Benchimol-Reis
• Modalidade de apoio: Auxílio à Pesquisa - Regular