Genetic deletion of IL-6 increases CK-MB, a classic cardiac damage marker, and decreases UPRmt genes after exhaustive exercise

Autor(es):Mostrar menos -

Rovina, Rafael L. ; Pinto, Ana P. ; Munoz, Vitor R. ; da Rocha, Alisson L. ; Rebelo, Macario A. ; Teixeira, Giovana R. ; Tavares, Maria Eduarda A. ; Pauli, Jose R. ; de Moura, Leandro P. ; Cintra, Dennys E. ; Ropelle, Eduardo R. ; da Silva, Adelino S. R.

• Tipo de documento: Artigo Científico
• Fonte: Cell Biochemistry and Function; v. 40, n. 4, p. 10-pg., 2022-04-12.

Resumo

The intensity, duration, type of contraction, and muscle damage influence interleukin-6 (IL-6) response to acute exercise. However, in response to an exhaustive exercise session, the upregulation of IL-6 in the serum and heart is associated with an inflammatory condition and can inhibit autophagy. This study aimed to investigate the role of IL-6 in autophagy pathway responses and mitochondrial function in the heart of mice submitted to acute exhaustive physical exercise. The mice were allocated into three groups, five animals per group, for the wild type (WT) and the IL-6 knockout (IL-6 KO): Basal (sedentary; Basal), 1 h (after 1 h of the acute exercise; 1 h), and 3 h (after 3 h of the acute exercise; 3 h). After the specific time for each group, the blood was collected, each mouse heart was removed, and the left ventricle (LV) was isolated. In summary, under basal conditions, without the influence of the acute exercise, the IL-6 KO group showed lower number of nuclei in the cardiac tissue, but higher collagen deposition; lower messenger RNA (mRNA) levels of Prkaa1 and Mtco1, but higher mRNA levels of Ulk1; and higher protein levels of the ratio p-AMPK/AMPK in the heart when compared to WT at the same time point. After the acute exercise (1 and 3 h), the IL-6 KO group had lower mRNA levels of Tfam, Mtnd1, Mtco1, and Nampt in the heart when compared to WT after exercise; higher serum levels of creatine kinase (CK), CK-MB, and lactate dehydrogenase for the IL-6 group when compared to the WT group after the exercise. Specifically, the heat-shock protein 60 protein levels in the heart increased 3 h after exhaustive exercise in the WT group, but not in the IL-6 KO group. The study emphasizes that IL-6 may offer cardioprotective effects, including mitochondrial adaptations in response to acute exhaustive exercise. (AU)

• Processo FAPESP: 17/19869-8 - Mecanismos moleculares relacionados ao aumento do conteúdo hepático de gordura em resposta ao excesso de exercício físico
• Beneficiário: Ana Paula Pinto
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 17/09038-1 - Efeitos do exercício físico regular e do overtraining no comportamento da via autofágica em diferentes tecidos de camundongos.
• Beneficiário: Adelino Sanchez Ramos da Silva
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 19/17058-8 - Papel da interleucina-6 nas respostas da via da autofagia no coração de camundongos submetidos ao exercício físico agudo exaustivo
• Beneficiário: Rafael Lemes Rovina
• Modalidade de apoio: Bolsas no Brasil - Mestrado
• Processo FAPESP: 17/12765-2 - Papel emergente da rev-erb-alfa nas adaptações moleculares a diferentes modelos de exercício físico
• Beneficiário: Alisson Luiz da Rocha
• Modalidade de apoio: Bolsas no Brasil - Doutorado