Effects of local and remote ischemic postconditioning methods on ischemiareperfusion injury in a young animal model of acute mesenteric ischemia

Autor(es)

Mateus Souza Abreu ^[1] ; Ana Cristina Aoun Tannuri ^[2] ; Rafael Felipe Gonçalves Rodrigues ^[3] ; Rafael José da Silva ^[4] ; Josiane de Oliveira Gonçalves ^[5] ; Suellen Serafini ^[6] ; Uenis Tannuri ^[7]

Afiliação do(s) autor(es)

^[1] Universidade de São Paulo. Faculdade de Medicina. Divisão de Cirurgia Pediátrica - Brasil

^[2] Universidade de São Paulo. Faculdade de Medicina. Divisão de Cirurgia Pediátrica - Brasil

^[3] Universidade de São Paulo. Faculdade de Medicina. Divisão de Cirurgia Pediátrica - Brasil

^[4] Universidade de São Paulo. Faculdade de Medicina. Divisão de Cirurgia Pediátrica - Brasil

^[5] Universidade de São Paulo. Faculdade de Medicina. Divisão de Cirurgia Pediátrica - Brasil

^[6] Universidade de São Paulo. Faculdade de Medicina. Divisão de Cirurgia Pediátrica - Brasil

^[7] Universidade de São Paulo. Faculdade de Medicina. Divisão de Cirurgia Pediátrica - Brasil

• Tipo de documento: Artigo Científico
• Fonte: Acta Cirurgica Brasileira; v. 38, 2023-06-02.

Resumo

ABSTRACT Purpose: Acute mesenteric ischemia (AMI) is a condition in pediatric surgery that ranges from intestine necrosis to death. Ischemic postconditioning (IPoC) methods were developed to reduce the damage caused by revascularization. This study aimed to evaluate the efficacy of these methods in an experimental weaning rat model. Methods: Thirty-two 21-day-old Wistar rats were allocated into four groups according to the surgical procedure performed: control, ischemia-reperfusion injury (IRI), local (LIPoC) and remote IPoC (RIPoC). At euthanasia, fragments of the intestine, liver, lungs, and kidneys were submitted to histological, histomorphometric, and molecular analyses. Results: In the duodenum, intestines, and kidneys histological alterations promoted by IRI were reversed by remote postconditioning method. In the distal ileum, the histomorphometric alterations could be reversed by the postconditioning methods with more evident effects promoted by the remote method. The molecular analysis found that the levels of expression of Bax (proapoptotic) and Bcl-XL (antiapoptotic) genes in the intestine were increased by IRI. These alterations were equally reversed by the postconditioning methods, with more evident effects of the remote method. Conclusions: IPoC methods positively reduced the damage caused by IRI in weaning rats. (AU)

• Processo FAPESP: 19/12465-4 - Avaliação histológica e molecular dos efeitos do pós-condicionamento isquêmico direto e remoto na isquemia intestinal aguda de ratos jovens
• Beneficiário: Mateus Souza Abreu
• Modalidade de apoio: Bolsas no Brasil - Iniciação Científica