| Texto completo | |
| Autor(es): |
Cezarette, G. N.
;
Sartim, M. A.
;
Sampaio, S. V.
Número total de Autores: 3
|
| Tipo de documento: | Artigo Científico |
| Fonte: | International Journal of Biological Macromolecules; v. 144, p. 9-pg., 2020-02-01. |
| Resumo | |
Inflammation and coagulopathies are important systemic events following snakebite. Snake venom galactoside-binding lectins (SVgalLs) are known modulators of the immune response with no direct effect on hemostasis. Considering the crosstalk between inflammation and coagulation, the present study investigated how BJcuL, a proinflammatory SVgalL isolated from Bothrops jararacussu venom, mediated the inflammation-induced procoagulant activity. We examined the proinflammatory cytokine production and procoagulant tissue factor (TF) activity in human whole blood and monocyte-rich cell suspension (MR-PBMC) treated with BJcuL. This lectin increased production of the cytokines TNF-alpha, IL-1 beta and IL-6, upregulated TF expression on the cell surface, and induced procoagulant activity. The proinflammatory behavior was mediated by the direct interaction between the lectin and toll-like receptor 4, via binding to beta-galactoside-containing glycoconjugates on the cell surface, and activation of NF kappa-B signaling. Interestingly, the BJcuL-induced inflammation was directly associated with the procoagulant activity of MR-PBMC cells. In whole blood culture, the lectin exhibited similar behavior, i.e. it induced cytokine production and MR-PBMC TF-mediated procoagulant activity. Therefore, the present study is the first report on the inflammation-induced procoagulant activity of SVgalLs, and it indicates that BJcuL is an important factor associated with coagulopathy in patients with snake envenomation. (C) 2019 Elsevier B.V. All rights reserved. (AU) | |
| Processo FAPESP: | 11/23236-4 - Toxinas animais nativas e recombinantes: análise funcional, estrutural e molecular |
| Beneficiário: | Suely Vilela |
| Modalidade de apoio: | Auxílio à Pesquisa - Temático |
| Processo FAPESP: | 15/06290-6 - Avaliação do potencial anti-inflamatório da crotoxina, uma fosfolipase A2 isolada da peçonha de Crotalus durissus terrificus, em modelo de coagulação intravascular disseminada induzida por endotoxemia |
| Beneficiário: | Marco Aurélio Sartim |
| Modalidade de apoio: | Bolsas no Brasil - Pós-Doutorado |
| Processo FAPESP: | 16/04079-9 - Avaliação do potencial anti-inflamatório da crotoxina, uma fosfolipase A2 isolada da peçonha de Crotalus durissus terrificus, em modelo de endotoxemia |
| Beneficiário: | Gabriel Neves Cezarette |
| Modalidade de apoio: | Bolsas no Brasil - Iniciação Científica |