Integrative microRNAome analysis of skeletal muscl... - BV FAPESP
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Integrative microRNAome analysis of skeletal muscle of Colossoma macropomum (tambaqui), Piaractus mesopotamicus (pacu), and the hybrid tambacu, based on next-generation sequencing data

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Autor(es):
Fantinatti, Bruno E. A. ; Perez, Erika S. ; Zanella, Bruna T. T. ; Valente, Jessica S. ; de Paula, Tassiana G. ; Mareco, Edson A. ; Carvalho, Robson F. ; Piazza, Silvano ; Denti, Michela A. ; Dal-Pai-Silva, Maeli
Número total de Autores: 10
Tipo de documento: Artigo Científico
Fonte: BMC Genomics; v. 22, n. 1, p. 17-pg., 2021-04-06.
Resumo

Background Colossoma macropomum (tambaqui) and Piaractus mesopotamicus (pacu) are good fish species for aquaculture. The tambacu, individuals originating from the induced hybridization of the female tambaqui with the male pacu, present rapid growth and robustness, characteristics which have made the tambacu a good choice for Brazilian fish farms. Here, we used small RNA sequencing to examine global miRNA expression in the genotypes pacu (PC), tambaqui (TQ), and hybrid tambacu (TC), (Juveniles, n = 5 per genotype), to better understand the relationship between tambacu and its parental species, and also to clarify the mechanisms involved in tambacu muscle growth and maintenance based on miRNAs expression. Results Regarding differentially expressed (DE) miRNAs between the three genotypes, we observed 8 upregulated and 7 downregulated miRNAs considering TC vs. PC; 14 miRNAs were upregulated and 10 were downregulated considering TC vs. TQ, and 15 miRNAs upregulated and 9 were downregulated considering PC vs. TQ. The majority of the miRNAs showed specific regulation for each genotype pair, and no miRNA were shared between the 3 genotype pairs, in both up- and down-regulated miRNAs. Considering only the miRNAs with validated target genes, we observed the miRNAs miR-144-3p, miR-138-5p, miR-206-3p, and miR-499-5p. GO enrichment analysis showed that the main target genes for these miRNAs were grouped in pathways related to oxygen homeostasis, blood vessel modulation, and oxidative metabolism. Conclusions Our global miRNA analysis provided interesting DE miRNAs in the skeletal muscle of pacu, tambaqui, and the hybrid tambacu. In addition, in the hybrid tambacu, we identified some miRNAs controlling important molecular muscle markers that could be relevant for the farming maximization. (AU)

Processo FAPESP: 16/05009-4 - Análise comparativa e integrativa do transcriptoma e microRNAoma do músculo esquelético de Colossoma macropomum (tambaqui), Piaractus mesopotamicus (pacu) e seu híbrido, tambacu: abordagens moleculares, in silico, in vitro e in vivo.
Beneficiário:Maeli Dal Pai
Modalidade de apoio: Auxílio à Pesquisa - Regular
Processo FAPESP: 15/19985-2 - Análise integrativa do microRNAoma no músculo esquelético de Colossoma macropomum (tambaqui), Piaractus mesopotamicus (pacu) e seu híbrido, tambacu
Beneficiário:Bruno Evaristo de Almeida Fantinatti
Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado
Processo FAPESP: 17/15708-0 - Bioinformática aplicada a microRNAs de peixes
Beneficiário:Bruno Evaristo de Almeida Fantinatti
Modalidade de apoio: Bolsas no Exterior - Estágio de Pesquisa - Pós-Doutorado