Impact of maternal protein restriction on Hypoxia-Inducible Factor (HIF) expression in male fetal kidney development

Autor(es)

Gomes, Julia Seva ; Sene, Leticia Barros ; Lamana, Gabriela Leme ; Boer, Patricia Aline ; Gontijo, Jose Antonio Rocha

• Tipo de documento: Artigo Científico
• Fonte: PLoS One; v. 18, n. 5, p. 18-pg., 2023-05-04.

Resumo

BackgroundKidney developmental studies have demonstrated molecular pathway changes that may be related to decreased nephron numbers in the male 17 gestational days (17GD) low protein (LP) intake offspring compared to normal protein intake (NP) progeny. Here, we evaluated the HIF-1 and components of its pathway in the kidneys of 17-GD LP offspring to elucidate the molecular modulations during nephrogenesis. MethodsPregnant Wistar rats were allocated into two groups: NP (regular protein diet-17%) or LP (Low protein diet-6%). Taking into account miRNA transcriptome sequencing previous study (miRNA-Seq) in 17GD male offspring kidneys investigated predicted target genes and proteins related to the HIF-1 pathway by RT-qPCR and immunohistochemistry. ResultsIn the present study, in male 17-GD LP offspring, an increased elF4, HSP90, p53, p300, NF kappa beta, and AT2 gene encoding compared to the NP progeny. Higher labeling of HIF-1 alpha CAP cells in 17-DG LP offspring was associated with reduced elF4 and phosphorylated elF4 immunoreactivity in LP progeny CAP cells. In 17DG LP, the NF kappa beta and HSP90 immunoreactivity was enhanced, particularly in the CAP area. Discussion and conclusionThe current study supported that the programmed reduced nephron number in the 17-DG LP offspring may be related to changes in the HIF-1 alpha signaling pathway. Factors that facilitate the transposition of HIF-1 alpha to progenitor renal cell nuclei, such as increased NOS, Ep300, and HSP90 expression, may have a crucial role in this regulatory system. Also, HIF-1 alpha changes could be associated with reduced transcription of elF-4 and its respective signaling path. (AU)

• Processo FAPESP: 05/54362-4 - Biologia do desenvolvimento renal em modelo de restrição proteica durante a gestação em ratas
• Beneficiário: Patrícia Aline Boer
• Modalidade de apoio: Auxílio à Pesquisa - Jovens Pesquisadores
• Processo FAPESP: 13/12486-5 - Programação fetal: sobre a ontogênese renal e neural
• Beneficiário: Jose Antonio Rocha Gontijo
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 14/50938-8 - INCT 2014: em Fotônica Aplicada à Biologia Celular INFABiC
• Beneficiário: Hernandes Faustino de Carvalho
• Modalidade de apoio: Auxílio à Pesquisa - Temático
• Processo FAPESP: 12/18492-4 - Estudo comparativo dos efeitos da restrição proteica in utero e da exposição à dexametasona in vitro durante a nefrogênese: avaliação da expressão gênica, de miRNAs, proteica e morfométrica no mesênquima metanefrogênico
• Beneficiário: Letícia de Barros Sene
• Modalidade de apoio: Bolsas no Brasil - Doutorado