Sonophotodynamic inactivation of Pseudomonas aeruginosa biofilm mediated by curcumin

Autor(es)

Silva e Carvalho, Iago ; Pratavieira, Sebastiao ; Bagnato, Vanderlei Salvador ; Alves, Fernanda

• Tipo de documento: Artigo Científico
• Fonte: BIOFOULING; v. 39, n. 6, p. 11-pg., 2023-07-26.

Resumo

The inactivation of Pseudomonas aeruginosa biofilm is a major challenge, as biofilms are less responsive to conventional treatments and responsible for persistent infections. This has led to the investigation of alternative approaches for biofilm control such as photodynamic (PDI) and sonodynamic (SDI) inactivation. The combination of them, known as Sonophotodynamic Inactivation (SPDI), has improved the effectiveness of the process. Curcumin, a well-established photosensitizer, has been identified as a potential sonosensitizer. This study evaluated the most effective combination for SPDI against P. aeruginosa biofilms in vitro, varying curcumin concentrations and ultrasound intensities. The results indicated that the inactivation was directly proportional to the curcumin concentration. Using curcumin 120 & mu;M and 3.0 W.cm(-2) of ultrasound intensity, SPDI demonstrated the highest and the best synergistic results, equivalent to 6.9 & PLUSMN; 2.1 logs of reduction. PDI reduced 0.7 & PLUSMN; 0.9 log and SDI had no effect. In conclusion, SPDI with curcumin is a promising approach for biofilm inactivation. (AU)

• Processo FAPESP: 13/07276-1 - CEPOF - Centro de Pesquisa em Óptica e Fotônica
• Beneficiário: Vanderlei Salvador Bagnato
• Modalidade de apoio: Auxílio à Pesquisa - Centros de Pesquisa, Inovação e Difusão - CEPIDs
• Processo FAPESP: 21/01324-0 - Desestruturação da matriz extracelular e uso de nanopartícula de curcumina para potencializar a inativação de biofilmes de Staphylococcus aureus
• Beneficiário: Fernanda Alves Dias de Sousa
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado
• Processo FAPESP: 14/50857-8 - INCT 2014 - de Óptica Básica e Aplicada às Ciências da Vida
• Beneficiário: Vanderlei Salvador Bagnato
• Modalidade de apoio: Auxílio à Pesquisa - Temático