Evaluation of cytotoxicity and genotoxicity of a novel oxovanadium complex with orotate

Autor(es)

da Silva Gomes, Pietra Stefany ; da Silva, Washington Wilson ; Gasparoti, Gabriela de Cassia ; Payolla, Filipe Boccato ; de Oliveira, Jonata Augusto ; Barbugli, Paula Aboud ; Marin-Dett, Freddy Humberto ; Cavicchioli, Mauricio ; Massabni, Antonio Carlos ; Resende, Flavia Aparecida

• Tipo de documento: Artigo Científico
• Fonte: MUTATION RESEARCH-GENETIC TOXICOLOGY AND ENVIRONMENTAL MUTAGENESIS; v. 883, p. 7-pg., 2022-11-01.

Resumo

The development of new drugs based on metal complexes requires a detailed analysis of their biological end-points. In this study, we report the genotoxic profile and influence on cell proliferation and death of the oxovanadium( IV) complex with orotic acid ([VO(C5H4N2O4)(2)], VO(oro)). Human hepatocellular carcinoma cells (HepG2) were the most sensitive tumor cells to VO(oro), which interfered with the integrity of cell membranes and proliferative capacity in a dose-dependent manner, inducing cell death by apoptosis. Regarding genotoxicity, VO(oro) did not induce considerable levels of DNA damage in HepG2 cells (comet test) and gene mutations (Ames test). However, it caused a statistically significant increase in the frequency of micronuclei at the highest concentration tested (12.5 mu mol.L-1), indicating aneuploidy and clastogenicity. The data presented here provide information on various biological aspects of the VO(oro) complex, which may allow the elucidation of its mechanism of action as a possible therapeutic agent. (AU)

• Processo FAPESP: 17/16278-9 - Estudo do potencial genotoxicológico e da permeabilidade em células Caco-2 de complexos metálicos com promissoras atividades biológicas
• Beneficiário: Flávia Aparecida Resende Nogueira
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 13/09265-7 - Avaliação do potencial de sistemas lipídicos nanoestruturados para administração de compostos de Cu(II) aplicáveis na otimização da terapia da tuberculose
• Beneficiário: Patrícia Bento da Silva
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado