Short-term strength exercise reduces the macrophage M1/M2 ratio in white adipose tissue of obese animals

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Fernandes, Celio Junior da Costa ; Rodrigues, Kellen Cristina da Cruz ; de Melo, Diego Gomes ; de Campos, Thais Dantis Pereira ; Canciglieri, Raphael dos Santos ; Simabuco, Fernando Moreira ; da Silva, Adelino Sanchez Ramos ; Cintra, Dennys Esper ; Ropelle, Eduardo Rochete ; Pauli, Jose Rodrigo ; de Moura, Leandro Pereira

• Tipo de documento: Artigo Científico
• Fonte: Life Sciences; v. 329, p. 10-pg., 2023-09-15.

Resumo

Obesity can exacerbate the systemic inflammatory process, leading to increased infiltration of monocytes in white adipose tissue (WAT) and polarization of these cells into pro-inflammatory M1 macrophages, while reducing the population of anti-inflammatory M2 macrophages. Aerobic exercise has been shown to be effective in reducing the pro-inflammatory profile. However, the impact of strength training and the duration of training on macrophage polarization in the WAT of obese individuals have not been widely studied. Therefore, our aim was to investigate the effects of resistance exercise on macrophage infiltration and polarization in the epididymal and subcutaneous adipose tissue of obese mice. We compared the following groups: Control (CT), Obese (OB), Obese 7-day strength training (STO7d), and Obese 15-day strength training (STO15d). Macrophage populations were evaluated by flow cytometry: total macrophages (F4/80+), M1 (CD11c), and M2 (CD206) macrophages. Our results demonstrated that both training protocols improved peripheral insulin sensitivity by increasing AKT phosphorylation (Ser473). Specifically, the 7-day training regimen reduced total macrophage infiltration and M2 macrophage levels without altering M1 levels. In the STO15d group, significant differences were observed in total macrophage levels, M1 macrophages, and the M1/M2 ratio compared to the OB group. In the epididymal tissue, a reduction in the M1/M2 ratio was observed in the STO7d group. Overall, our data demonstrate that 15 days of strength exercise can reduce the M1/M2 ratio of macrophages in white adipose tissue. (AU)

• Processo FAPESP: 21/00880-7 - Efeitos do jejum e do exercício físico de força na via de sinalização AMPK / TBK1 no fígado de camundongos obesos e diabéticos
• Beneficiário: Leandro Pereira de Moura
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 21/02541-5 - Efeitos do exercício aeróbio na expressão/função da MTCH2 hepática: um estudo da bancada ao leito para o controle da EHNA
• Beneficiário: Célio Junior da Costa Fernandes
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado
• Processo FAPESP: 19/16012-4 - Os efeitos do exercício aeróbio sobre a função e expressão hepática da MTCH2: um estudo da bancada ao leito para o controle da DHGNA
• Beneficiário: Leandro Pereira de Moura
• Modalidade de apoio: Auxílio à Pesquisa - Regular