Intracellular peptides in SARS-CoV-2-infected patients

Autor(es)

Martucci, Luiz Felipe ; Eichler, Rosangela A. S. ; Silva, Renee N. O. ; Costa, Tiago J. ; Tostes, Rita C. ; Busatto, Geraldo F. ; Seelaender, Marilia C. L. ; Duarte, Alberto J. S. ; Souza, Heraldo P. ; Ferro, Emer S.

• Tipo de documento: Artigo Científico
• Fonte: ISCIENCE; v. 26, n. 9, p. 17-pg., 2023-09-15.

Resumo

Intracellular peptides (InPeps) generated by the orchestrated action of the proteasome and intracellular peptidases have biological and pharmacological sig-nificance. Here, human plasma relative concentration of specific InPeps was compared between 175 patients infected with severe acute respiratory syn-drome coronavirus 2 (SARS-CoV-2), and 45 SARS-CoV-2 non-infected patients; 2,466 unique peptides were identified, of which 67% were InPeps. The results re-vealed differences of a specific group of peptides in human plasma comparing non-infected individuals to patients infected by SARS-CoV-2, following the re-sults of the semi-quantitative analyses by isotope-labeled electrospray mass spectrometry. The protein-protein interactions networks enriched pathways, drawn by genes encoding the proteins from which the peptides originated, re-vealed the presence of the coronavirus disease/COVID-19 network solely in the group of patients fatally infected by SARS-CoV-2. Thus, modulation of the rela-tive plasma levels of specific InPeps could be employed as a predictive tool for disease outcome. (AU)

• Processo FAPESP: 13/08216-2 - CPDI - Centro de Pesquisa em Doenças Inflamatórias
• Beneficiário: Fernando de Queiroz Cunha
• Modalidade de apoio: Auxílio à Pesquisa - Centros de Pesquisa, Inovação e Difusão - CEPIDs
• Processo FAPESP: 16/04000-3 - Farmacologia de oligopeptidases e peptídeos intracelulares
• Beneficiário: Emer Suavinho Ferro
• Modalidade de apoio: Auxílio à Pesquisa - Temático
• Processo FAPESP: 17/25116-2 - Papel da O-GlcNAcilação (O-GlcNAc) na expressão e função do receptor de estrogênio alfa clássico (ERa66kDa) e do splice variant do receptor de estrogênio alfa (ERa36kDa) em artéria carótida comum de camundongos envelhecidos
• Beneficiário: Tiago Januário da Costa
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado