Meta-omics integration approach reveals the effect... - BV FAPESP
Busca avançada
Ano de início
Entree


Meta-omics integration approach reveals the effect of soil native microbiome diversity in the performance of inoculant Azospirillum brasilense

Texto completo
Autor(es):
Ferrarezi, Jessica Aparecida ; Defant, Heloisa ; de Souza, Leandro Fonseca ; Azevedo, Joao Lucio ; Hungria, Mariangela ; Quecine, Maria Carolina
Número total de Autores: 6
Tipo de documento: Artigo Científico
Fonte: FRONTIERS IN PLANT SCIENCE; v. 14, p. 15-pg., 2023-06-29.
Resumo

Plant growth promoting bacteria (PGPB) have been used as integrative inputs to minimize the use of chemical fertilizers. However, a holistic comprehension about PGPB-plant-microbiome interactions is still incipient. Furthermore, the interaction among PGPB and the holobiont (host-microbiome association) represent a new frontier to plant breeding programs. We aimed to characterize maize bulk soil and rhizosphere microbiomes in irradiated soil (IS) and a native soil (NS) microbial community gradient (dilution-to-extinction) with Azospirillum brasilense Ab-V5, a PGPB commercial inoculant. Our hypothesis was that plant growth promotion efficiency is a result of PGPB niche occupation and persistence according to the holobiont conditions. The effects of Ab-V5 and NS microbial communities were evaluated in microcosms by a combined approach of microbiomics (species-specific qPCR, 16S rRNA metataxonomics and metagenomics) and plant phenomics (conventional and high-throughput methods). Our results revealed a weak maize growth promoting effect of Ab-V5 inoculation in undiluted NS, contrasting the positive effects of NS dilutions 10(-3), 10(-6), 10(-9) and IS with Ab-V5. Alpha diversity in NS + Ab-V5 soil samples was higher than in all other treatments in a time course of 25 days after sowing (DAS). At 15 DAS, alpha diversity indexes were different between NS and IS, but similar in all NS dilutions in rhizospheric samples. These differences were not persistent at 25 DAS, demonstrating a stabilization process in the rhizobiomes. In NS 10(-3) +Ab-V5 and NS 10(-6) Ab-V5, Ab-V5 persisted in the maize rhizosphere until 15 DAS in higher abundances compared to NS. In NS + Ab-V5, abundance of six taxa were positively correlated with response to (a)biotic stresses in plant-soil interface. Genes involved in bacterial metabolism of riboses and amino acids, and cresol degradation were abundant on NS 10(-3) + Ab-V5, indicating that these pathways can contribute to plant growth promotion and might be a result of Ab-V5 performance as a microbial recruiter of beneficial functions to the plant. Our results demonstrated the effects of holobiont on Ab-V5 performance. The meta-omics integration supported by plant phenomics opens new perspectives to better understanding of inoculants-holobiont interaction and for developing better strategies for optimization in the use of microbial products. (AU)

Processo FAPESP: 19/25720-2 - Desvendando o papel de bactérias promotoras de crescimento em plantas no recrutamento do microbioma da rizosfera do milho
Beneficiário:Jessica Aparecida Ferrarezi
Modalidade de apoio: Bolsas no Brasil - Doutorado Direto
Processo FAPESP: 19/04697-2 - Estabelecimento de uma comunidade sintética de bactérias promotoras de crescimento de planta e a sua associação genética com um painel de diversidade de milho tropical
Beneficiário:João Lúcio de Azevedo
Modalidade de apoio: Auxílio à Pesquisa - Regular
Processo FAPESP: 21/11983-1 - Construção de consórcio bacteriano para milho: estudo da interação, promoção de crescimento e modulação da expressão gênica
Beneficiário:Heloísa Defant
Modalidade de apoio: Bolsas no Brasil - Mestrado