| Texto completo | |
| Autor(es): |
Scaramboni, Caroline
;
Campos, Maria Lucia Arruda Moura
;
Dorta, Daniel Junqueira
;
Oliveira, Danielle Palma de
;
Medeiros, Silvia Regina Batistuzzo de
;
Galva, Marcos Felipe de Oliveira
;
Dreij, Kristian
Número total de Autores: 7
|
| Tipo de documento: | Artigo Científico |
| Fonte: | TOXICOLOGY IN VITRO; v. 91, p. 9-pg., 2023-06-14. |
| Resumo | |
Retene is a polycyclic aromatic hydrocarbon (PAH) emitted mainly by biomass combustion, and despite its ubiquity in atmospheric particulate matter (PM), studies concerning its potential hazard to human health are still incipient. In this study, the cytotoxicity and genotoxicity of retene were investigated in human HepG2 liver cells. Our data showed that retene had minimal effect on cell viability, but induced DNA strand breaks, micronuclei formation, and reactive oxygen species (ROS) formation in a dose-and time-dependent manner. Stronger effects were observed at earlier time points than at longer, indicating transient genotoxicity. Retene activated phos-phorylation of Checkpoint kinase 1 (Chk1), an indicator of replication stress and chromosomal instability, which was in accordance with increased formation of micronuclei. A protective effect of the antioxidant N-acetylcys-teine (NAC) towards ROS generation and DNA damage signaling was observed, suggesting oxidative stress as a key mechanism of the observed genotoxic effects of retene in HepG2 cells. Altogether our results suggest that retene may contribute to the harmful effects caused by biomass burning PM and represent a potential hazard to human health. (AU) | |
| Processo FAPESP: | 18/16554-9 - Avaliando o impacto das emissões atmosféricas relacionadas à produção e uso de etanol na perspectiva das atuais políticas públicas de biocombustíveis |
| Beneficiário: | Maria Lúcia Arruda de Moura Campos |
| Modalidade de apoio: | Auxílio à Pesquisa - Regular |
| Processo FAPESP: | 18/17931-0 - Material particulado atmosférico de uma região impactada pela queima de biomassa: fontes e toxicidade |
| Beneficiário: | Caroline Scaramboni |
| Modalidade de apoio: | Bolsas no Brasil - Doutorado |
| Processo FAPESP: | 14/50945-4 - INCT 2014: Instituto Nacional de Tecnologias Alternativas para Detecção, Avaliação Toxicológica e Remoção de Micropoluentes e Radioativos |
| Beneficiário: | Maria Valnice Boldrin |
| Modalidade de apoio: | Auxílio à Pesquisa - Temático |