Eight Weeks of Physical Training Decreases 2 Years of DNA Methylation Age of Sedentary Women

Autor(es)

Rodrigues, Guilherme da Silva ; Noma, Isabella Harumi Yonehara ; Noronha, Natalia Yumi ; Watanabe, Ligia Moriguchi ; Sobrinho, Andressa Crystine da Silva ; de Lima, Joao Gabriel Ribeiro ; Sae-Lee, Chanachai ; Benjamim, Cicero Jonas Rodrigues ; Nonino, Carla Barbosa ; Bueno Jubuir, Carlos Roberto

• Tipo de documento: Artigo Científico
• Fonte: RESEARCH QUARTERLY FOR EXERCISE AND SPORT; v. N/A, p. 11-pg., 2023-07-21.

Resumo

Purpose: The acceleration of epigenetic age is a predictor of mortality and contributes to the increase in chronic diseases. Adherence to a healthy lifestyle is a strategy to reduce epigenetic age. The present study aimed to determine whether eight weeks of combined (aerobic and strength) training (CT) can influence the epigenetic age of women between 50 and 70 years old and the differences in sites and methylated regions. Methods: Eighteen women (AAR(Low): lower age acceleration residual, n = 10; AAR(High): higher age acceleration residual, n = 8) participated in a combined exercise training program (60 minutes, 3x a week) for eight weeks. DNA was extracted from whole blood using the salting out technique. DNA methylation was performed using the array technique (Illumina's Infinium Methylation BeadChip 850k). We used the DNA Methylation Age Calculator platform to calculate the biological epigenetic age. Two-way ANOVA followed by FISHER LSD posthoc was Applied, adopting p < .05. Results: After eight weeks of CT, there were no changes to the epigenetic age acceleration for the AAR(Low) group (PRE: -2.3 & PLUSMN; 3.2 to POST: -2.3 & PLUSMN; 3.6). However, the AAR(High) group significantly decreased the age acceleration (PRE: 3.6 & PLUSMN; 2.6 to POST: 2.2 & PLUSMN; 2.7) (group effect, p = .01; time effect, p = .31; group vs. time effect, p = .005). Conclusion: CT for eight weeks benefits the epigenetic clock of women with the most accelerated age. (AU)

• Processo FAPESP: 17/21361-2 - Screening global de fatores genéticos e epigenéticos associados com magnitude de resposta a diferentes treinamentos físicos em mulheres de 50 a 70 anos
• Beneficiário: Carlos Roberto Bueno Júnior
• Modalidade de apoio: Auxílio à Pesquisa - Regular