Mining reactive triplet carbonyls in biological systems

Autor(es)

Ramos, Luiz D. ; Gomes, Thiago M. V. ; V. Stevani, Cassius ; Bechara, Etelvino J. H.

• Tipo de documento: Artigo Científico
• Fonte: JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY B-BIOLOGY; v. 243, p. 16-pg., 2023-06-01.

Resumo

Aliphatic triplet carbonyls can be treated as short-lived radicals, since both species share similar reactions such as hydrogen atom abstraction, cyclization, addition, and isomerization. Importantly, enzyme-generated triplet carbonyls excite triplet molecular oxygen to the highly reactive, electrophilic singlet state by resonance energy transfer, which can react with proteins, lipids, and DNA. Carbonyl triplets, singlet oxygen, and radicals are endowed with the potential to trigger both normal and pathological responses. In this paper, we present a short review of easy, fast, and inexpensive preliminary tests for the detection of transient triplet carbonyls in chemical and biological systems. This paper covers direct and indirect methods to look for triplet carbonyls based on their spectral distribution of chemiluminescence, photoproduct analysis, quenching of light emission by conjugated dienes, and enhancement of light emission by the sensitizer 9,10-dibromoanthracence-2-sulfonate ion (DBAS). (AU)

• Processo FAPESP: 17/22501-2 - Quimiexcitação eletrônica em sistemas biológicos: bioluminescência e 'foto'química no escuro
• Beneficiário: Etelvino José Henriques Bechara
• Modalidade de apoio: Auxílio à Pesquisa - Temático
• Processo FAPESP: 22/03864-5 - Supressão de metabólitos carbonílicos no estado triplete por sorbatos alquilados, esterificados e derivados peptídicos
• Beneficiário: Thiago da Mata Viola Gomes
• Modalidade de apoio: Bolsas no Brasil - Doutorado Direto
• Processo FAPESP: 19/24515-6 - Fontes, alvos e respostas biológicas de espécies tripletes e oxigênio singlete: patogenicidade e supressão
• Beneficiário: Luiz Duarte Ramos
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado