The effect of ultrasound on improving the extraction of tannins from the Stryphnodendron adstringens bark

Autor(es)

Ribeiro, Mauricio M. de Souza ; Vigano, Juliane ; de Novais, Nathalia Stahl ; Mesquita, Leonardo M. de Souza ; Kamikawachi, Renan Canute ; Vilegas, Wagner ; Lopes, Patricia Santos ; da Silva, Camila Soares ; Rostagno, Mauricio Ariel ; Veggi, Priscila Carvalho

• Tipo de documento: Artigo Científico
• Fonte: SUSTAINABLE CHEMISTRY AND PHARMACY; v. 33, p. 14-pg., 2023-03-10.

Resumo

Stryphnodendron adstringens (Barbatimao) barks is one of the plants listed in the national list of medicinal plants of interest to the Brazilian health system and in the Brazilian pharmacopeia. Its extracts have been used in commercial products and popular medicine due to their phenolic compound's composition. Reported works dealing with Barbatimao and ultrasound-assisted extraction (UAE) does not cover all possible variables to be studied. Also, there is a gap in understanding the ultrasound phenomena's effect on the extract composition and biological properties of the Barbatimao. This work aimed to apply the UAE, using hydroethanolic solution (50 wt%) as the solvent to recover phenolic compounds from Barbatimao barks. Amplitude (0-40%), solvent-to-feed mass ratio (5-25), and extraction time (2-12 min) were evaluated. Ferric-reducing antioxidant capacity (FRAP), antioxidant cell activity, and cytotoxicity were also evaluated. The results showed that UAE parameters play significant roles in obtaining higher yields of phenolic compounds compared to other processes including conventional techniques. It was identified that the ultrasound waves contribute to enhancing the tannin extraction through mechanisms that only ultrasound, among the tested techniques, can provide. The extract obtained by UAE promoted cell viability higher than 80%, while the extracts obtained by the conventional method did not demonstrate this activity. UAE showed an efficient technique to obtain a tannins-rich extract from Barbatimaobarks. (AU)

• Processo FAPESP: 20/15774-5 - Desenvolvimento de métodos de extração, separação e análise bidimensional de compostos fenólicos em produtos naturais
• Beneficiário: Juliane Viganó
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado
• Processo FAPESP: 18/14582-5 - Desenvolvimento de um sistema bidimensional de extração, separação e análise para avaliação da bioatividade de fitoquímicos
• Beneficiário: Maurício Ariel Rostagno
• Modalidade de apoio: Auxílio à Pesquisa - Jovens Pesquisadores - Fase 2
• Processo FAPESP: 19/13496-0 - EMU aprovado no processo 2018/14582-5: sistema de cromatografia de ultra alta pressão (UHPLC), composto por bomba quaternária, injetor automático, forno com seletor de coluna, detector PDA
• Beneficiário: Maurício Ariel Rostagno
• Modalidade de apoio: Auxílio à Pesquisa - Programa Equipamentos Multiusuários
• Processo FAPESP: 20/07575-2 - Aplicação de tecnologias de alta pressão para obtenção de bioprodutos: montagem de um equipamento multipropósito e desenvolvimento de processos para o barbatimão
• Beneficiário: Priscilla Carvalho Veggi
• Modalidade de apoio: Auxílio à Pesquisa - Regular