Bovine blastocyst-like structures derived from stem cell cultures

Autor(es):Mostrar menos -

Pinzon-Arteaga, Carlos A. ; Wang, Yinjuan ; Wei, Yulei ; Orsi, Ana E. Ribeiro ; Li, Leijie ; Scatolin, Giovanna ; Liu, Lizhong ; Sakurai, Masahiro ; Ye, Jianfeng ; Ming, Hao ; Yu, Leqian ; Li, Bo ; Jiang, Zongliang ; Wu, Jun

• Tipo de documento: Artigo Científico
• Fonte: CELL STEM CELL; v. 30, n. 5, p. 14-pg., 2023-05-04.

Resumo

Understanding the mechanisms of blastocyst formation and implantation is critical for improving farm animal reproduction but is hampered by a limited supply of embryos. Here, we developed an efficient method to generate bovine blastocyst-like structures (termed blastoids) via assembling bovine trophoblast stem cells and expanded potential stem cells. Bovine blastoids resemble blastocysts in morphology, cell composition, single-cell transcriptomes, in vitro growth, and the ability to elicit maternal recognition of pregnancy following transfer to recipient cows. Bovine blastoids represent an accessible in vitro model for studying embryogen-esis and improving reproductive efficiency in livestock species. (AU)

• Processo FAPESP: 22/02096-4 - Análise do status de inativação do cromossomo X em blastóides humanos
• Beneficiário: Ana Elisa Ribeiro Orsi
• Modalidade de apoio: Bolsas no Exterior - Estágio de Pesquisa - Mestrado
• Processo FAPESP: 20/16612-9 - Análise da expressão gênica de embriões humanos pré-implantação para o desenvolvimento de estratégia de manutenção do estado epigenético da mórula
• Beneficiário: Ana Elisa Ribeiro Orsi
• Modalidade de apoio: Bolsas no Brasil - Mestrado