Citral Modulates MMP-2 and MMP-9 Activities on Healing of Gastric Ulcers Associated with High-Fat Diet-Induced Obesity

Autor(es)

Ohara, Rie ; Dario, Felipe Lima ; Emilio-Silva, Maycon Tavares ; Assuncao, Renata ; Rodrigues, Vinicius Peixoto ; Bueno, Gabriela ; Raimundo, Priscila Romano ; da Rocha, Lucia Regina Machado ; Hiruma-Lima, Clelia Akiko

• Tipo de documento: Artigo Científico
• Fonte: INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES; v. 24, n. 5, p. 15-pg., 2023-03-01.

Resumo

Obesity causes low-grade inflammation that results in the development of comorbidities. In people with obesity, exacerbation of gastric lesion severity and delayed healing may aggravate gastric mucosal lesions. Accordingly, we aimed to evaluate the citral effects on gastric lesion healing in eutrophic and obese animals. C57Bl/6 male mice were divided into two groups: animals fed a standard diet (SD) or high-fat diet (HFD) for 12 weeks. Gastric ulcers were induced using acetic acid (80%) in both groups. Citral (25, 100, or 300 mg/kg) was administered orally for 3 or 10 days. A vehicle-treated negative control (1% Tween 80, 10 mL/kg) and lansoprazole-treated (30 mg/kg) were also established. Lesions were macroscopically examined by quantifying regenerated tissue and ulcer areas. Matrix metalloproteinases (MMP-2 and -9) were analyzed by zymography. The ulcer base area between the two examined periods was significantly reduced in HFD 100 and 300 mg/kg citral-treated animals. In the 100 mg/kg citral-treated group, healing progression was accompanied by reduced MMP-9 activity. Accordingly, HFD could alter MMP-9 activity, delaying the initial healing phase. Although macroscopic changes were undetectable, 10-day treatment with 100 mg/kg citral exhibited improved scar tissue progression in obese animals, with reduced MMP-9 activity and modulation of MMP-2 activation. (AU)

• Processo FAPESP: 18/09873-0 - Obesidade induzida por dieta hipercalórica: avaliação da ação do Citral na inflamação metabólica, sistêmica e na cicatrização das úlceras gástricas
• Beneficiário: Clélia Akiko Hiruma Lima
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 20/07384-2 - A cicatrização gástrica pode ser alterada em animais obesos? Avaliação do efeito antiulcerogênico do citral em camundongos tratados com dieta hiperlipídica
• Beneficiário: Felipe Lima Dario
• Modalidade de apoio: Bolsas no Brasil - Iniciação Científica
• Processo FAPESP: 20/15225-1 - Avaliação da ação do citral na permeabilidade intestinal e endotoxemia metabólica em camundongos obesos alimentados com dieta hiperlipídica
• Beneficiário: Maycon Tavares Emílio Silva
• Modalidade de apoio: Bolsas no Brasil - Doutorado