Toxicological effects of cyanobacterial metabolites on zebrafish larval development

Autor(es)

Jacinavicius, Fernanda R. ; Geraldes, Vanessa ; Fernandes, Kelly ; Crnkovic, Camila M. ; Gama, Watson A. ; Pinto, Ernani

• Tipo de documento: Artigo Científico
• Fonte: HARMFUL ALGAE; v. 125, p. 16-pg., 2023-03-31.

Resumo

Freshwater cyanobacteria are known worldwide for their potential to produce toxins. However, these organisms are also found in marine, terrestrial and extreme environments and produce unique compounds, other than toxins. Nevertheless, their effects on biological systems are still barely known. This work tested extracts of different cyanobacterial strains against zebrafish (Danio rerio) larvae and analyzed their metabolomic profiles using liquid chromatography combined with mass spectrometry. Strains Desertifilum tharense, Anagnostidinema amphibium, and Nostoc sp. promoted morphological abnormalities such as pericardial edema, edema in the digestive system region, curvature of the tail and spine in zebrafish larvae in vivo. In contrast, Microcystis aer-uginosa and Chlorogloeopsis sp. did not promote such changes. Metabolomics revealed unique compounds belonging to the classes of terpenoids, peptides, and linear lipopeptides/microginins in the nontoxic strains. The toxic strains were shown to contain unique compounds belonging to the classes of cyclic peptides, amino acids and other peptides, anabaenopeptins, lipopeptides, terpenoids, and alkaloids and derivatives. Other unknown compounds were also detected, highlighting the rich structural diversity of secondary metabolites produced by cyanobacteria. The effects of cyanobacterial metabolites on living organisms, mainly those related to potential human and ecotoxicological risks, are still poorly known. This work highlights the diverse, complex, and unique metabolomic profiles of cyanobacteria and the biotechnological potential and associated risks of exposure to their metabolites. (AU)

• Processo FAPESP: 20/07710-7 - Prospecção biotecnológica de produtos naturais a partir de cianobactérias
• Beneficiário: Camila Manoel Crnkovic
• Modalidade de apoio: Auxílio à Pesquisa - Programa BIOTA - Regular
• Processo FAPESP: 19/27707-3 - Implementação do serviço de proteômica baseada em espectrometria de massas no FoRC
• Beneficiário: Vanessa Sofia Mendes Geraldes
• Modalidade de apoio: Bolsas no Brasil - Programa Capacitação - Treinamento Técnico
• Processo FAPESP: 21/00149-0 - Tratamento e fracionamento seletivo de cianobactérias em florações para produção de insumos de valor agregado
• Beneficiário: Ernani Pinto Junior
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 13/07914-8 - FoRC - Centro de Pesquisa em Alimentos
• Beneficiário: Bernadette Dora Gombossy de Melo Franco
• Modalidade de apoio: Auxílio à Pesquisa - Centros de Pesquisa, Inovação e Difusão - CEPIDs