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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Actinobacillus actinomycetemcomitans-induced periodontal disease in mice: patterns of cytokine, chemokine, and chemokine receptor expression and leukocyte migration

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Autor(es):
Garlet, Gustavo P. [1] ; Avila-Campos, Mario J. ; Milanezi, Cristiane M. ; Fereira, Beatriz R. ; Silva, João S.
Número total de Autores: 5
Afiliação do(s) autor(es):
[1] Universidade de São Paulo (USP). Faculdade de Medicina de Ribeirão Preto. Departamento de Bioquímica e Imunologia - Brasil
Número total de Afiliações: 5
Tipo de documento: Artigo Científico
Fonte: Microbes and Infection; v. 7, n. 4, p. 738-747, Apr. 2004.
Área do conhecimento: Ciências da Saúde - Odontologia
Assunto(s):Periodontia   Patologia bucal
Resumo

Although the pathogenesis of periodontal disease (PD) is not well known, cytokines, chemotactic factors and inflammatory cells are certainly involved in the disease outcome. Here, we characterized the evolution of the PD induced by Actinobacillus actinomycetemcomitans in mice, showing that oral inoculation of these bacteria leads to the migration of leukocytes to periodontal tissues and marked alveolar bone resorption. We found the expression of pro-inflammatory and Th1-type cytokines including TNF-a, IFN-? and IL-12 in periodontal tissues after infection with A. actinomycetemcomitans, from the early stages after infection and throughout the course of the disease. Similar kinetics of expression were found for the chemokines CCL5, CCL4, CCL3 and CXCL10 and for the receptors CCR5 and CXCR3, all of them linked to the Th1-type pattern. The expression of the Th2-type mediators IL-10, CCL1 and their receptors CCR4 and CCR8 was detected only after 30 days of infection, determining a time-dependent mixed pattern of polarized immune response. The chemokine expression was correlated with the presence of polymorphonuclear leukocytes, macrophages, CD4 and CD8 lymphocytes, and B cells in the inflammatory infiltrate. Interestingly, during the predominance of the Th1-type response, a sharp increase in the number of inflammatory cells and intense bone loss was seen. By contrast, after the increased expression of Th2-type mediators, the number of inflammatory cells remained constant. Our data demonstrate that mice subjected to oral inoculation of A. actinomycetemcomitans represent a useful model for the study of PD. In addition, our results suggest that expression of cytokines and chemokines can drive the selective recruitment of leukocyte subsets to periodontal tissues, which could determine the stable or progressive nature of the lesion. (AU)

Processo FAPESP: 04/06731-8 - Modulação da resposta imune em doenças periodontais induzidas experimentalmente
Beneficiário:João Santana da Silva
Linha de fomento: Auxílio à Pesquisa - Regular
Processo FAPESP: 01/09998-7 - Estudo do papel do IFN-gamma, TNF-algha, IL-4 e iNOS na imunomodulação da doença periodontal
Beneficiário:Gustavo Pompermaier Garlet
Linha de fomento: Bolsas no Brasil - Doutorado