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A Novel Chemically Modified Curcumin Reduces Severity of Experimental Periodontal Disease in Rats: Initial Observations

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Autor(es):
Elburki, Muna S. ; Rossa, Carlos ; Guimaraes, Morgana R. ; Goodenough, Mark ; Lee, Hsi-Ming ; Curylofo, Fabiana A. ; Zhang, Yu ; Johnson, Francis ; Golub, Lorne M.
Número total de Autores: 9
Tipo de documento: Artigo Científico
Fonte: Mediators of Inflammation; v. 2014, p. 10-pg., 2014-01-01.
Resumo

Tetracycline-based matrix metalloproteinase-(MMP-) inhibitors are currently approved for two inflammatory diseases, periodontitis and rosacea. The current study addresses the therapeutic potential of a novel pleiotropic MMP-inhibitor not based on an antibiotic. To induce experimental periodontitis, endotoxin (LPS) was repeatedly injected into the gingiva of rats on one side of the maxilla; the contralateral (control) side received saline injections. Two groups of rats were treated by daily oral intubation with a chemically modified curcumin, CMC 2.24, for two weeks; the control groups received vehicle alone. After sacrifice, gingiva, blood, and maxilla were collected, the jaws were defleshed, and periodontal (alveolar) bone loss was quantified morphometrically and by mu-CT scan. The gingivae were pooled per experimental group, extracted, and analyzed for MMPs (gelatin zymography; western blot) and for cytokines (e.g., IL-1 beta; ELISA); serum and plasma samples were analyzed for cytokines and MMP-8. The LPS-induced pathologically excessive bone loss was reduced to normal levels based on either morphometric (P = 0.003) or mu-CT (P = 0.008) analysis. A similar response was seen for MMPs and cytokines in the gingiva and blood. This initial study, on a novel triketonic zinc-binding CMC, indicates potential efficacy on inflammatory mediators and alveolar bone loss in experimental periodontitis and warrants future therapeutic and pharmacokinetic investigations. (AU)

Processo FAPESP: 10/19660-2 - Mecanismos biológicos da modulação de osteoclastogênese, reabsorção e reparo ósseo mediados por curcumin
Beneficiário:Morgana Rodrigues Guimarães Stabili
Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado
Processo FAPESP: 12/15826-9 - Modulação da reabsorção óssea inflamatória por curcumin modificado quimicamente: efeito na osteoclastogênese
Beneficiário:Fabiana Almeida Curylofo Zotti
Modalidade de apoio: Bolsas no Brasil - Mestrado
Processo FAPESP: 09/54080-0 - EMU: aquisição de aparelho de micro tomografia in vivo para o Laboratório de Caracterização e Avaliação de Resposta Biológica
Beneficiário:Elcio Marcantonio Junior
Modalidade de apoio: Auxílio à Pesquisa - Programa Equipamentos Multiusuários