Evaluation of Lasting Effects of Heat Stress on Sperm Profile and Oxidative Status of Ram Semen and Epididymal Sperm

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dos Santos Hamilton, Thais Rose ; Mendes, Camilla Mota ; de Castro, Leticia Signori ; de Assis, Patricia Monken ; Perez Siqueira, Adriano Felipe ; Delgado, Juliana de Carvalho ; Goissis, Marcelo Demarchi ; Muino-Blanco, Teresa ; Alvaro Cebrian-Perez, Jose ; Nichi, Marcilio ; Visintin, Jose Antonio ; Ortiz D'Avila Assumpcao, Mayra Elena

• Tipo de documento: Artigo Científico
• Fonte: OXIDATIVE MEDICINE AND CELLULAR LONGEVITY; v. 2016, p. 12-pg., 2016-01-01.

Resumo

Higher temperatures lead to an increase of testicular metabolism that results in spermatic damage. Oxidative stress is the main factor responsible for testicular damage caused by heat stress. The aim of this study was to evaluate lasting effects of heat stress on ejaculated sperm and immediate or long-term effects of heat stress on epididymal sperm. We observed decrease in motility and mass motility of ejaculated sperm, as well as an increase in the percentages of sperm showing major and minor defects, damaged plasma and acrosome membranes, and a decrease in the percentage of sperm with high mitochondrial membrane potential in the treated group until one spermatic cycle. An increased enzymatic activity of glutathione peroxidase and an increase of stressed cells were observed in ejaculated sperm of the treated group. A decrease in the percentage of epididymal sperm with high mitochondrial membrane potential was observed in the treated group. However, when comparing immediate and long-term effects, we observed an increase in the percentage of sperm with low mitochondrial membrane potential. In conclusion, testicular heat stress induced oxidative stress that led to rescuable alterations after one spermatic cycle in ejaculated sperm and also after 30 days in epididymal sperm. (AU)

• Processo FAPESP: 07/58487-1 - Influencia dos indices espermaticos de protamina sobre a fertilidade in vitro em bovinos.
• Beneficiário: Mayra Elena Ortiz D' Avila Assumpcao
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 13/05025-1 - Mensuração das enzimas antioxidantes no sêmen de ovinos: concentrações plasmáticas e celulares
• Beneficiário: Thais Rose dos Santos Hamilton
• Modalidade de apoio: Bolsas no Exterior - Estágio de Pesquisa - Doutorado
• Processo FAPESP: 11/11231-8 - Estudo dos fatores envolvidos na fragmentação de DNA dos espermatozóides em ovinos.
• Beneficiário: Thais Rose dos Santos Hamilton
• Modalidade de apoio: Bolsas no Brasil - Doutorado