| Texto completo | |
| Autor(es): |
Rodrigues, Bruno V. M.
;
Leite, Nelly C. S.
;
Cavalcanti, Bruno das Neves
;
da Silva, Newton S.
;
Marciano, Fernanda R.
;
Corat, Evaldo J.
;
Webster, Thomas J.
;
Lobo, Anderson O.
Número total de Autores: 8
|
| Tipo de documento: | Artigo Científico |
| Fonte: | INTERNATIONAL JOURNAL OF NANOMEDICINE; v. 11, p. 17-pg., 2016-01-01. |
| Resumo | |
Nanohydroxyapatite (nHAp) is an emergent bioceramic that shows similar chemical and crystallographic properties as the mineral phase present in bone. However, nHAp presents low fracture toughness and tensile strength, limiting its application in bone tissue engineering. Conversely, multi-walled carbon nanotubes (MWCNTs) have been widely used for composite applications due to their excellent mechanical and physicochemical properties, although their hydrophobicity usually impairs some applications. To improve MWCNT wettability, oxygen plasma etching has been applied to promote MWCNT exfoliation and oxidation and to produce graphene oxide (GO) at the end of the tips. Here, we prepared a series of nHAp/MWCNT-GO nanocomposites aimed at producing materials that combine similar bone characteristics (nHAp) with high mechanical strength (MWCNT-GO). After MWCNT production and functionalization to produce MWCNT-GO, ultrasonic irradiation was employed to precipitate nHAp onto the MWCNT-GO scaffolds (at 1-3 wt%). We employed various techniques to characterize the nanocomposites, including transmission electron microscopy (TEM), Raman spectroscopy, thermogravimetry, and gas adsorption (the Brunauer-Emmett-Teller method). We used simulated body fluid to evaluate their bioactivity and human osteoblasts (bone-forming cells) to evaluate cytocompatibility. We also investigated their bactericidal effect against Staphylococcus aureus and Escherichia coli. TEM analysis revealed homogeneous distributions of nHAp crystal grains along the MWCNT-GO surfaces. All nanocomposites were proved to be bioactive, since carbonated nHAp was found after 21 days in simulated body fluid. All nanocomposites showed potential for biomedical applications with no cytotoxicity toward osteoblasts and impressively demonstrated a bactericidal effect without the use of antibiotics. All of the aforementioned properties make these materials very attractive for bone tissue engineering applications, either as a matrix or as a reinforcement material for numerous polymeric nanocomposites. (AU) | |
| Processo FAPESP: | 15/08523-8 - Desenvolvimento de um nanosensor fluorescente a partir da eletrofiação de PVA e quantum dots de grafeno: aplicação na detecção de biomarcadores de Alzheimer |
| Beneficiário: | Bruno Vinícius Manzolli Rodrigues |
| Modalidade de apoio: | Bolsas no Brasil - Pós-Doutorado |
| Processo FAPESP: | 11/20345-7 - Estudo de filmes de carbono-tipo diamante contendo nanopartículas incorporadas para aplicações biomédicas |
| Beneficiário: | Fernanda Roberta Marciano |
| Modalidade de apoio: | Auxílio à Pesquisa - Jovens Pesquisadores |
| Processo FAPESP: | 13/20054-8 - Estudos de adesão, proliferação e de morte celular na interação de células com materiais nanoestruturados |
| Beneficiário: | Newton Soares da Silva |
| Modalidade de apoio: | Auxílio à Pesquisa - Regular |
| Processo FAPESP: | 11/17877-7 - Desenvolvimento de novos scaffolds poliméricos por eletrofiação com incorporação de nanotubos alinhados e nanohidroxiapatita para regeneração óssea |
| Beneficiário: | Anderson de Oliveira Lobo |
| Modalidade de apoio: | Auxílio à Pesquisa - Jovens Pesquisadores |