Overexpression of mir-183 and mir-494 promotes proliferation and migration in human breast cancer cell lines

Autor(es)

Macedo, Taciane ; Silva-Oliveira, Renato J. ; Silva, Viviane A. O. ; Vidal, Daniel O. ; Evangelista, Adriane F. ; Marques, Marcia M. C.

• Tipo de documento: Artigo Científico
• Fonte: Oncology Letters; v. 14, n. 1, p. 7-pg., 2017-07-01.

Resumo

Breast cancer (BC) is a leading cause of cancer-associated mortality in females worldwide. MicroRNAs (miRNAs or miRs), a type of non-coding RNA, have been reported to be important in the regulation of BC onset and progression. Several studies have implicated the role of miR-183 and miR-494 in different types of cancer. However, the biological functions of these miRNAs in BC remain largely unknown. In the present study, the expression of both miRNAs was assessed in the MDA-MB-231 and MDA-MB-468 BC cell lines. It was hypothesized that miR-183 and miR-494 serve an important role in regulating the expression of key genes associated with the metastatic phenotype of BC cells. To further understand their role, the expression of these miRNAs was restored in selected BC cell lines. Functional assays revealed that overexpression of miR-183 or miR-494 modulated the proliferation and migration of MDA-MB-231 and MDA-MB-468 cells in vitro. Additionally, retinoblastoma 1 (RB1) was identified to be a downstream target of both miRNAs by in silico analysis. Western blotting revealed that upregulation of miR-183 was associated with downregulation of RB1 protein in MDA-MB-231 cells. In conclusion, the present results support the hypothesis that miR-183 and miR-494 serve a pivotal role in BC metastasis, and that miR-183 may act as an oncogene by targeting RB1 protein in MDA-MB-231 cells. (AU)

• Processo FAPESP: 10/16796-0 - Avaliação do papel de microRNAs diferencialmente expressos no controle de invasão e metástase do câncer de mama
• Beneficiário: Márcia Maria Chiquitelli Marques Silveira
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 12/17111-7 - Estudo funcional de microRNAs associados ao processo de metástase em linhagens celulares de carcinoma mamário humano
• Beneficiário: Taciane Macedo
• Modalidade de apoio: Bolsas no Brasil - Mestrado