Skin Wound Healing Potential and Mechanisms of the Hydroalcoholic Extract of Leaves and Oleoresin of Copaifera langsdorffii Desf. Kuntze in Rats

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Sergio Gushiken, Lucas Fernando ; Hussni, Carlos Alberto ; Bastos, Jairo Kenupp ; Rozza, Ariane Leite ; Beserra, Fernando Pereira ; Vieira, Ana Julia ; Padovani, Carlos Roberto ; Lemos, Marivane ; Polizello Junior, Maurilio ; Mangabeira da Silva, Jonas Joaquim ; Nobrega, Rafael Henrique ; Monteiro Martinez, Emanuel Ricardo ; Pellizzon, Claudia Helena

• Tipo de documento: Artigo Científico
• Fonte: Evidence-based Complementary and Alternative Medicine; v. 2017, p. 16-pg., 2017-01-01.

Resumo

The wound healing is a complex process which, sometimes, can be a problem in public health because of the possibility of physical disability or even death. Due to the lack of a gold standard drug in skin wound treatment and aiming at the discovery of new treatments in skin repair and the mechanisms involved in the process, we used oleoresin (OR) from Copaifera langsdorffii and hydroalcoholic extract of the leaves (EH) to treat rat skin wounds. For that, male Wistar rats were divided into groups (n = 8): Lanette, Collagenase, 10% EH, or 10% OR and, after anesthesia, one wound of 2 cm was made in the back of animals. The wounds were treated once a day for 3, 7, or 14 days and the wound areas were measured. The rats were euthanized and skin samples destined to biochemical, molecular, and immunohistochemical analysis. The results showed a macroscopic retraction of the wounds of 10% EH and 10% OR creams and both treatments showed anti-inflammatory activity. Molecular and immunohistochemical results demonstrated the activity of Copaifera langsdorffii creams in angiogenesis, reepithelialization, wound retraction, and remodeling mechanisms. (AU)

• Processo FAPESP: 11/13630-7 - Validação química e farmacológica de extratos e princípios ativos de espécies de Copaifera
• Beneficiário: Jairo Kenupp Bastos
• Modalidade de apoio: Auxílio à Pesquisa - Temático
• Processo FAPESP: 14/23684-5 - Avaliação dos mecanismos celulares e moleculares na cicatrização de lesões cutâneas utilizando cremes à base de extrato hidroalcoólico e óleo-resina de Copaifera langsdorffii
• Beneficiário: Lucas Fernando Sérgio Gushiken
• Modalidade de apoio: Bolsas no Brasil - Mestrado
• Processo FAPESP: 12/15092-5 - Avaliação do processo de cicatrização de lesões cutâneas utilizando extrato hidroalcóolico e óleo de copaíba
• Beneficiário: Lucas Fernando Sérgio Gushiken
• Modalidade de apoio: Bolsas no Brasil - Iniciação Científica