| Texto completo | |
| Autor(es): Mostrar menos - |
Polaquini, Carlos R.
;
Morao, Luana G.
;
Nazare, Ana C.
;
Torrezan, Guilherme S.
;
Dilarri, Guilherme
;
Cavalca, Lucia B.
;
Campos, Debora L.
;
Silva, Isabel C.
;
Pereira, Jesse A.
;
Scheffers, Dirk-Jan
;
Duque, Cristiane
;
Pavan, Fernando R.
;
Ferreira, Henrique
;
Regasini, Luis O.
Número total de Autores: 14
|
| Tipo de documento: | Artigo Científico |
| Fonte: | BIOORGANIC CHEMISTRY; v. 90, p. 8-pg., 2019-09-01. |
| Resumo | |
Curcumin is a plant diphenylheptanoid and has been investigated for its antibacterial activity. However, the therapeutic uses of this compound are limited due to its chemical instability. In this work, we evaluated the antimicrobial activity of diphenylheptanoids derived from curcumin against Gram-positive and Gram-negative bacteria, and also against Mycobacterium tuberculosis in terms of MIC (Minimum Inhibitory Concentration) and MBC (Minimum Bactericidal Concentration) values. 3,3'-Dihydroxycurcumin (DHC) displayed activity against Enterococcus faecalis, Staphylococcus aureus and M. tuberculosis, demonstrating MIC values of 78 and 156 mu g/mL. In addition, DHC was more stable than curcumin in acetate buffer (pH 5.0) and phosphate buffer (pH 7.4) for 24 h at 37 degrees C. We proposed that membrane and the cell division protein FtsZ could be the targets for DHC due to that fact that curcumin exhibits this mode of antibacterial action. Fluorescence microscopy of Bacillus subtilis stained with SYTO9 and propidium iodide fluorophores indicated that DHC has the ability to perturb the bacterial membrane. On the other hand, DHC showed a weak inhibition of the GTPase activity of B. subtilis FtsZ. Toxicity assay using human cells indicated that DHC has moderate capacity to reduce viability of liver cells (HepG2 line) and lung cells (MRC-5 and A549 lines) when compared with doxorubicin. Alkaline comet assay indicated that DHC was not able to induce DNA damage in A549 cell line. These results indicated that DHC is promising compound with antibacterial and antitubercular activities. (AU) | |
| Processo FAPESP: | 09/53989-4 - EMU: aquisição de espectrômetro de ressonância magnética nuclear para estudos de biomoléculas |
| Beneficiário: | Raghuvir Krishnaswamy Arni |
| Modalidade de apoio: | Auxílio à Pesquisa - Programa Equipamentos Multiusuários |
| Processo FAPESP: | 13/50367-8 - New environmental-friendly compounds to combat Citrus canker |
| Beneficiário: | Henrique Ferreira |
| Modalidade de apoio: | Auxílio à Pesquisa - Regular |
| Processo FAPESP: | 15/50162-2 - Proteção das plantas com peptídeos antimicrobianos e galatos - Pro-Planta |
| Beneficiário: | Henrique Ferreira |
| Modalidade de apoio: | Auxílio à Pesquisa - Temático |
| Processo FAPESP: | 15/08089-6 - Preparação e Avaliação Biológica de Diidroxibenzoatos de Alquila (DA) como Agentes Úteis ao Combate do Cancro Cítrico |
| Beneficiário: | Ana Carolina Nazaré |
| Modalidade de apoio: | Bolsas no Brasil - Doutorado |
| Processo FAPESP: | 17/50216-0 - Uso de rejeitos agrícolas para o combate de fitopatógenos: formas ambientalmente amigáveis para combater o Xanthomonas citri |
| Beneficiário: | Henrique Ferreira |
| Modalidade de apoio: | Auxílio à Pesquisa - Regular |
| Processo FAPESP: | 14/50880-0 - INCT 2014: de genômica comparativa e funcional e melhoramento assistido de citros |
| Beneficiário: | Marcos Antonio Machado |
| Modalidade de apoio: | Auxílio à Pesquisa - Temático |
| Processo FAPESP: | 18/15083-2 - Síntese e avaliação biológica de isobavachalcona (IBC) e análogos como potenciais agentes contra a tuberculose |
| Beneficiário: | Luis Octávio Regasini |
| Modalidade de apoio: | Auxílio à Pesquisa - Regular |