Cell wall hydrolases act in concert during aerenchyma development in sugarcane roots

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Grandis, Adriana ; Leite, Debora C. C. ; Tavares, Eveline Q. P. ; Arenque-Musa, Bruna C. ; Gaiarsa, Jonas W. ; Martins, Marina C. M. ; De Souza, Amanda P. ; Gomez, Leonardo D. ; Fabbri, Claudia ; Mattei, Benedetta ; Buckeridge, Marcos S.

• Tipo de documento: Artigo Científico
• Fonte: ANNALS OF BOTANY; v. 124, n. 6, p. 23-pg., 2019-11-04.

Resumo

Background and Aims Cell wall disassembly occurs naturally in plants by the action of several glycosyl-hydrolases during different developmental processes such as lysigenous and constitutive aerenchyma formation in sugarcane roots. Wall degradation has been reported in aerenchyma development in different species, but little is known about the action of glycosyl-hydrolases in this process. Methods In this work, gene expression, protein levels and enzymatic activity of cell wall hydrolases were assessed. Since aerenchyma formation is constitutive in sugarcane roots, they were assessed in segments corresponding to the first 5 cm from the root tip where aerenchyma develops. Key Results Our results indicate that the wall degradation starts with a partial attack on pectins (by acetyl esterases, endopolygalacturonases, beta-galactosidases and alpha-arabinofuranosidases) followed by the action of beta-glucan-/callose-hydrolysing enzymes. At the same time, there are modifications in arabinoxylan (by alpha-arabinofuranosidases), xyloglucan (by XTH), xyloglucan-cellulose interactions (by expansins) and partial hydrolysis of cellulose. Saccharification revealed that access to the cell wall varies among segments, consistent with an increase in recalcitrance and composite formation during aerenchyma development. Conclusion Our findings corroborate the hypothesis that hydrolases are synchronically synthesized, leading to cell wall modifications that are modulated by the fine structure of cell wall polymers during aerenchyma formation in the cortex of sugarcane roots. (AU)

• Processo FAPESP: 09/52840-7 - Centro de Processos Biológicos e Industriais para Biocombustíveis - CeProBIO
• Beneficiário: Igor Polikarpov
• Modalidade de apoio: Auxílio à Pesquisa - Programa BIOEN - Temático
• Processo FAPESP: 10/17104-5 - Degradação da parede celular em raízes de cana-de-açúcar
• Beneficiário: Marcos Silveira Buckeridge
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 08/57908-6 - Instituto Nacional de Biotecnologia para o Bioetanol
• Beneficiário: Marcos Silveira Buckeridge
• Modalidade de apoio: Auxílio à Pesquisa - Programa BIOEN - Temático
• Processo FAPESP: 14/50884-5 - INCT 2014: Instituto Nacional de Ciência e Tecnologia do Bioetanol
• Beneficiário: Marcos Silveira Buckeridge
• Modalidade de apoio: Auxílio à Pesquisa - Temático
• Processo FAPESP: 10/17070-3 - Determinação das enzimas de degradação da parede celular durante a formação do aerênquima em raízes de cana de açúcar
• Beneficiário: Adriana Grandis
• Modalidade de apoio: Bolsas no Brasil - Doutorado