| Texto completo | |
| Autor(es): |
Migliorini, Fernanda L.
;
dos Santos, Danilo M.
;
Soares, Andrey C.
;
Mattoso, Luiz H. C.
;
Oliveira Jr, Osvaldo N.
;
Correa, Daniel S.
Número total de Autores: 6
|
| Tipo de documento: | Artigo Científico |
| Fonte: | CHEMOSENSORS; v. 8, n. 3, p. 14-pg., 2020-09-01. |
| Resumo | |
We report a paper-based electrochemical immunosensor made with sustainable materials to detect aflatoxin B1 (AFB1), a highly toxic, carcinogenic mycotoxin found in food. The immunosensor was prepared with a waterproof paper substrate and low-cost graphite-based conductive ink through a simple cut-printing method. The working electrode was functionalized with a drop-cast film of multiwalled carbon nanotubes (MWCNT)/chitosan on which a layer of anti-AFB1 monoclonal antibodies was immobilized covalently. The architecture of the immunosensor was confirmed with polarization-modulated infrared reflection absorption spectroscopy (PM-IRRAS) and electrochemical impedance spectroscopy (EIS), including the effective immobilization of the active layer of anti-AFB1. With EIS as the principle of detection, the immunosensor could detect AFB1 in the range from 1 to 30 ng center dot mL(-1), and detection limit of 0.62 ng center dot mL(-1). This sensitivity is sufficient to detect AFB1 in food according to regulatory agencies. The immunosensor exhibited good repeatability, reproducibility, stability, and selectivity in experiments with a possible interferent. Furthermore, detection of AFB1 in maize flour samples yielded recovery of 97-99%, in a demonstration of the possible use of the paper-based immunosensor to detect AFB1 using extraction solutions from food samples. (AU) | |
| Processo FAPESP: | 17/20973-4 - Nanofibras eletrofiadas coaxiais à base de quitosana para a liberação controlada de antibióticos e aplicações no tratamento de lesões periodontais |
| Beneficiário: | Danilo Martins dos Santos |
| Modalidade de apoio: | Bolsas no Brasil - Pós-Doutorado |
| Processo FAPESP: | 18/22214-6 - Rumo à convergência de tecnologias: de sensores e biossensores à visualização de informação e aprendizado de máquina para análise de dados em diagnóstico clínico |
| Beneficiário: | Osvaldo Novais de Oliveira Junior |
| Modalidade de apoio: | Auxílio à Pesquisa - Temático |
| Processo FAPESP: | 18/18953-8 - Filmes nanoestruturados aplicados em biossensores microfluídicos para detecção de mastite bacteriana |
| Beneficiário: | Andrey Coatrini Soares |
| Modalidade de apoio: | Bolsas no Brasil - Pós-Doutorado |
| Processo FAPESP: | 17/21791-7 - Desenvolvimento de biossensores eletroquímicos e colorimétricos para a detecção de micotoxinas |
| Beneficiário: | Fernanda Lanzoni Migliorini |
| Modalidade de apoio: | Bolsas no Brasil - Pós-Doutorado |