Deleterious effects of gestational diabetes mellitus on the characteristics of the rectus abdominis muscle associated with pregnancy-specific urinary incontinence

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Vesentini, Giovana ; Barbosa, Angelica M. P. ; Floriano, Juliana F. ; Felisbino, Sergio L. ; Costa, Sarah M. B. ; Piculo, Fernanda ; Marini, Gabriela ; Nunes, Sthefanie K. ; Reyes, David R. A. ; Marcondes, Joao P. C. ; Hallur, Raghavendra L. S. ; Rozza, Ariane L. ; Magalhaes, Claudia G. ; Costa, Roberto ; Abbade, Joelcio F. ; Corrente, Jose E. ; Calderon, Iracema M. P. ; Matheus, Selma M. M. ; Rudge, Marilza V. C. ; Diamater Study Grp

• Tipo de documento: Artigo Científico
• Fonte: Diabetes Research and Clinical Practice; v. 166, p. 12-pg., 2020-08-01.

Resumo

Aims: To evaluate the effects of gestational diabetes mellitus (GDM) on the structural characteristics of the rectus abdominis muscle (RAM) and its indirect effects on pregnancyspecific urinary incontinence (PSUI). Methods: A total of 92 pregnant women were divided into four groups, according to their clinical conditions: non-GDM continent, non-GDM associated PSUI, GDM continent and GDM associated PSUI. The muscle morphometry (histochemistry and immunohistochemistry) for the fiber types and collagen fiber distribution, the ultrastructural analysis (transmission electron microscopy), the protein expression of fiber types and calcium signaling (Western blotting), and the content of types I and III collagen fiber (ELISA) in RAM collected at delivery were assessed. Results: The GDM groups presented a significantly increased number of slow fibers and slow-twitch oxidative fiber expression; decreased fiber area, number of fast fibers, and area of collagen; an increase in central nuclei; ultrastructural alterations with focal lesion areas such as myeloid structures, sarcomere disorganization, and mitochondrial alteration. The PSUI groups presented a considerable decrease in types I and III collagen contents and the localization of collagen fiber. Conclusions: Our data reveal that GDM causes morphological, biochemical and physiologi-cal changes in the RAM, and this might predispose women to PSUI. (C) 2020 Elsevier B.V. All rights reserved. (AU)

• Processo FAPESP: 14/26852-6 - Repercussões da hiperglicemia gestacional e incontinência urinária gestacional no músculo reto abdominal: matriz extracelular, expressão proteica e ultraestrutura
• Beneficiário: Giovana Vesentini
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 16/09710-9 - Caracterização de marcadores do binômio hiperglicemia gestacional e incontinência urinária gestacional na predição de incontinência urinária 6-12 meses pós-parto
• Beneficiário: Marilza Vieira Cunha Rudge
• Modalidade de apoio: Auxílio à Pesquisa - Regular