The role of nanohydroxyapatite on the morphological, physical, and biological properties of chitosan nanofibers

Autor(es)

Sato, Tabata P. ; Rodrigues, Bruno V. M. ; Mello, Daphne C. R. ; Munchow, Eliseu A. ; Ribeiro, Juliana S. ; Machado, Joao Paulo B. ; Vasconcellos, Luana M. R. ; Lobo, Anderson O. ; Bottino, Marco C. ; Borges, Alexandre L. S.

• Tipo de documento: Artigo Científico
• Fonte: CLINICAL ORAL INVESTIGATIONS; v. 25, n. 5, p. 9-pg., 2020-10-13.

Resumo

Objectives This study aimed to evaluate the effects of nanohydroxyapatite (nHAp) particles on the morphological, chemical, physical, and biological properties of chitosan electrospun nanofibers. Materials and methods nHAp particles with a 1.67 Ca/P ratio were synthesized via the aqueous precipitation method, incorporated into chitosan polymer solution (0.5 wt%), and electrospun into nHAp-loaded fibers (ChHa fibers). Neat chitosan fibers (nHAp-free, Ch fibers) were used as the control. The electrospun fiber mats were characterized using morphological, topographical, chemical, thermal, and a range of biological (antibacterial, antibiofilm, cell viability, and alkaline phosphatase [ALP] activity) analyses. Data were analyzed using ANOVA and Tukey's test (alpha= 0.05). Results ChHa fibers demonstrated a bead-like morphology, with thinner (331 +/- 110 nm) and smoother (Ra = 2.9 +/- 0.3 mu m) distribution as compared to the control fibers. Despite showing similar cell viability and ALP activity to Ch fibers, the ChHa fibers demonstrated greater antibacterial potential against most tested bacteria (except forP. intermedia), and higher antibiofilm activity againstP. gingivalisbiofilm. Conclusions The incorporation of nHAp particles did not jeopardize the overall morphology, topography, physical, and biological characteristics of the chitosan nanofibers. (AU)

• Processo FAPESP: 11/20345-7 - Estudo de filmes de carbono-tipo diamante contendo nanopartículas incorporadas para aplicações biomédicas
• Beneficiário: Fernanda Roberta Marciano
• Modalidade de apoio: Auxílio à Pesquisa - Jovens Pesquisadores
• Processo FAPESP: 11/17877-7 - Desenvolvimento de novos scaffolds poliméricos por eletrofiação com incorporação de nanotubos alinhados e nanohidroxiapatita para regeneração óssea
• Beneficiário: Anderson de Oliveira Lobo
• Modalidade de apoio: Auxílio à Pesquisa - Jovens Pesquisadores