Cytotoxicity of water-soluble fraction from biodiesel and its diesel blends to human cell lines

Autor(es)

Leme, Daniela Morais ^[1] ; Grummt, Tamara ^[2] ; Heinze, Rita ^[2] ; Sehr, Andrea ^[2] ; Skerswetat, Matthias ^[2] ; Rodrigues de Marchi, Mary Rosa ^[3] ; Machado, Marcos Canto ^[3] ; de Oliveira, Danielle Palma ^[4] ; Mann-Morales, Maria Aparecida ^[1]

Afiliação do(s) autor(es)

^[1] Univ Estadual Paulista UNESP, Dept Biol, BR-13506900 Rio Claro, SP - Brazil

^[2] Fed Environm Agcy, Bad Elster Branch, D-08645 Bad Elster - Germany

^[3] Univ Estadual Paulista UNESP, Inst Chem, BR-14800900 Araraquara, SP - Brazil

^[4] USP, Fac Ciencias Farmaceut Ribeirao Preto, Dept Anal Clin Toxicol & Bromatol, BR-14040903 Ribeirao Preto, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY; v. 74, n. 8, p. 2148-2155, NOV 2011.
• Citações Web of Science: 14

Resumo

The designation of biodiesel as a green fuel has increased its commercialization and use, making its fate in the environment a matter of concern. Fuel spills constitute a major source of aquatic pollution and, like diesel spills, biodiesel can produce adverse effects on aquatic environments, animals and humans. The present study assessed cytotoxic effects of water systems contaminated with neat biodiesel and its diesel blends by means of different procedures on human T cell leukemia (Jurkat) and human hepatocellular carcinoma (HepG2) cells {[}detection of changes in mitochondrial membrane potential (Delta Psi(m)) using tetramethylrhodamine ethyl ester (TMRE), apoptosis recognition by Annexin V and impedance real-time cell analyzer (xCELLigence (TM) system)]. The data obtained showed concordance across the different bioassays, with cytotoxic effects observed as a dose-dependent response only for waters contaminated with pure diesel (D100) and B5 blend, which is characterized by a mixture of 95% diesel and 5% biodiesel. The data can also lead us to hypothesize that diesel accounts for the harmful effects observed, and that biodiesel does not worsen the impacts caused by diesel pollution. (C) 2011 Elsevier Inc. All rights reserved. (AU)

• Processo FAPESP: 07/04304-3 - Avaliação da genotoxicidade e mutagenicidade de misturas comerciais de diesel e biodiesel puras e em simulações de vazamentos em água e em solo
• Beneficiário: Daniela Morais Leme
• Modalidade de apoio: Bolsas no Brasil - Doutorado