Aerobic growth physiology of Saccharomyces cerevisiae on sucrose is strain-dependent

Autor(es)

Soares Rodrigues, Carla Ines ; Wahl, Aljoscha ; Gombert, Andreas K.

• Tipo de documento: Artigo Científico
• Fonte: FEMS Yeast Research; v. 21, n. 3, p. 13-pg., 2021-04-07.

Resumo

Present knowledge on the quantitative aerobic physiology of the yeast Saccharomyces cerevisiae during growth on sucrose as sole carbon and energy source is limited to either adapted cells or to the model laboratory strain CEN.PK113-7D. To broaden our understanding of this matter and open novel opportunities for sucrose-based biotechnological processes, we characterized three strains, with distinct backgrounds, during aerobic batch bioreactor cultivations. Our results reveal that sucrose metabolism in S. cerevisiae is a strain-specific trait. Each strain displayed distinct extracellular hexose concentrations and invertase activity profiles. Especially, the inferior maximum specific growth rate (0.21 h(-1)) of the CEN.PK113-7D strain, with respect to that of strains UFMG-CM-Y259 (0.37 h(-1)) and JP1 (0.32 h(-1)), could be associated to its low invertase activity (0.04-0.09 U/mg(DM)). Moreover, comparative experiments with glucose or fructose alone, or in combination, suggest mixed mechanisms of sucrose utilization by the industrial strain JP1, and points out the remarkable ability of the wild isolate UFMG-CM-259 to grow faster on sucrose than on glucose in a well-controlled cultivation system. This work hints to a series of metabolic traits that can be exploited to increase sucrose catabolic rates and bioprocess efficiency. (AU)

• Processo FAPESP: 16/07285-9 - Por que Saccharomyces cerevisiae não apresenta velocidade específica de crescimento maior em glicose do que em sacarose?
• Beneficiário: Carla Ines Soares Rodrigues
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 17/18206-5 - Levedura turbo: altas velocidades glicolíticas apresentadas por Saccharomyces cerevisiae em sacarose
• Beneficiário: Carla Ines Soares Rodrigues
• Modalidade de apoio: Bolsas no Exterior - Estágio de Pesquisa - Doutorado
• Processo FAPESP: 17/08464-7 - Metabolismo de sacarose em Saccharomyces cerevisiae: a caminho de leveduras sintéticas com maiores fluxos glicolíticos para a biotecnologia industrial
• Beneficiário: Andreas Karoly Gombert
• Modalidade de apoio: Auxílio à Pesquisa - Programa BIOEN - Regular