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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Molecular cytogenetic analysis of Haemulidae fish evolutionary conservation

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Autor(es):
Motta Neto, Clovis Coutinho [1] ; Cioffi, Marcelo Belo [2] ; Carlos Bertollo, Luis Antonio [2] ; Molina, Wagner Franco [1]
Número total de Autores: 4
Afiliação do(s) autor(es):
[1] Univ Fed Rio Grande do Norte, Ctr Biociencias, Dept Biol Celular & Genet, BR-59078970 Natal, RN - Brazil
[2] Univ Fed Sao Carlos, Dept Genet & Evolucao, BR-13560 Sao Carlos, SP - Brazil
Número total de Afiliações: 2
Tipo de documento: Artigo Científico
Fonte: Journal of Experimental Marine Biology and Ecology; v. 407, n. 1, p. 97-100, OCT 15 2011.
Citações Web of Science: 18
Resumo

Several fish species belonging to the family Haemulidae present a karyotype consisting of 48 acrocentric chromosomes (FN = 48), and apparently similar chromosomal microstructure, especially in genus Haemulon, representing a striking example of intrafamiliar chromosomal conservation. In this study, a more detailed cytogenetic analysis of the species Conodon nobilis and Pomadasys corvinaeformis was performed using C-banding, Ag-NOR, DAPI/CMA(3) staining, in situ digestion by distinct endonucleases and double-FISH to map the 18S and 5S ribosomal genes. Both species showed a similar karyotypic macrostructure with 2n = 48 acrocentric chromosomes and active ribosomal sites at interstitial position on long arms of chromosomal pair 18 and 24 in P. corvinaeformis and C. nobilis, respectively. These sites were the only CMA(3)(+)/DAPI-regions in the karyotype. Digestion with restriction enzymes revealed a low number of digestion sites in the heterochromatic segments of both species. The data indicate some degree of interspecific evolutionary diversification At the microstructural level, incorporated in a general pattern of extensive karyotypic conservatism. Thus, the interspecific reproductive isolation leading to phyletic diversification apparently occurred without the contribution of conspicuous karyotypic changes. (C) 2011 Elsevier B.V. All rights reserved. (AU)

Processo FAPESP: 09/14881-3 - Evolução cromossômica na família Erythrinidae: mapeamento citogenético de DNAs repetitivos e microdissecção de cromossomos sexuais
Beneficiário:Marcelo de Bello Cioffi
Linha de fomento: Bolsas no Brasil - Doutorado