| Texto completo | |
| Autor(es): |
Ferreira, Rodolfo Sanches
;
Jandrey, Elisa Helena Farias
;
Granha, Isabela
;
Endo, Alice Kei
;
Ferreira, Raiane Oliveira
;
Araujo, Bruno Henrique Silva
;
Zatz, Mayana
;
Okamoto, Oswaldo Keith
Número total de Autores: 8
|
| Tipo de documento: | Artigo Científico |
| Fonte: | Viruses-Basel; v. 16, n. 11, p. 14-pg., 2024-11-01. |
| Resumo | |
Central nervous system (CNS) cancers are responsible for high rates of morbidity and mortality worldwide. Malignant CNS tumors such as adult Glioblastoma (GBM) and pediatric embryonal CNS tumors such as medulloblastoma (MED) and atypical teratoid rhabdoid tumors (ATRT) present relevant therapeutic challenges due to the lack of response to classic treatment regimens with radio and chemotherapy. Recent findings on the Zika virus' (ZIKV) ability to infect and kill CNS neoplastic cells draw attention to the virus' oncolytic potential. Studies demonstrating the safety of using ZIKV for treating malignant CNS tumors, enabling the translation of this approach to clinical trials, are scarce in the literature. Here we developed a co-culture model of mature human cerebral organoids assembled with GBM, MED or ATRT tumor cells and used these assembloids to test ZIKV oncolytic effect, replication potential and preferential targeting between normal and cancer cells. Our hybrid co-culture models allowed the tracking of tumor cell growth and invasion in cerebral organoids. ZIKV replication and ensuing accumulation in the culture medium was higher in organoids co-cultured with tumor cells than in isolated control organoids without tumor cells. ZIKV infection led to a significant reduction in tumor cell proportion in organoids with GBM and MED cells, but not with ATRT. Tumoroids (3D cultures of tumor cells alone) were efficiently infected by ZIKV. Interestingly, ZIKV rapidly replicated in GBM, MED, and ATRT tumoroids reaching significantly higher viral RNA accumulation levels than co-cultures. Moreover, ZIKV infection reduced viable cells number in MED and ATRT tumoroids but not in GBM tumoroids. Altogether, our findings indicate that ZIKV has greater replication rates in aggressive CNS tumor cells than in normal human cells comprising cerebral organoids. However, such higher ZIKV replication in tumor cells does not necessarily parallels oncolytic effects, suggesting cellular intrinsic and extrinsic factors mediating tumor cell death by ZIKV. (AU) | |
| Processo FAPESP: | 19/27784-8 - Estudo de vírus oncolítico em modelo in vitro de glioblastoma desenvolvido em organóide cerebral |
| Beneficiário: | Rodolfo Sanches Ferreira |
| Modalidade de apoio: | Bolsas no Brasil - Mestrado |
| Processo FAPESP: | 23/02209-6 - Análise do efeito oncolítico do ZIKV em células de glioblastoma resistentes a temozolomida |
| Beneficiário: | Isabela Fonseca de Oliveira Granha |
| Modalidade de apoio: | Bolsas no Brasil - Mestrado |
| Processo FAPESP: | 20/14109-8 - Estudo do efeito oncolítico do Vírus da Zika nas células do microambiente de tumores do sistema nervoso central |
| Beneficiário: | Raiane de Oliveira Ferreira |
| Modalidade de apoio: | Bolsas no Brasil - Doutorado Direto |
| Processo FAPESP: | 21/04323-5 - Avaliação da expressão e atividade de EZH2 como fatores de susceptibilidade celular à infecção por Zika Vírus em tumores embrionários do sistema nervoso central |
| Beneficiário: | Elisa Helena Farias Jandrey |
| Modalidade de apoio: | Bolsas no Brasil - Pós-Doutorado |
| Processo FAPESP: | 13/08028-1 - CEGH-CEL - Centro de Estudos do Genoma Humano e de Células-Tronco |
| Beneficiário: | Mayana Zatz |
| Modalidade de apoio: | Auxílio à Pesquisa - Centros de Pesquisa, Inovação e Difusão - CEPIDs |