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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Molecular characterization of Hepatozoon sp in cats from Sao Luis Island, Maranhao, Northeastern Brazil

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Autor(es):
de Bortoli, Caroline P. [1] ; Andre, Marcos R. [1] ; Braga, Maria do Socorro C. [1, 2] ; Machado, Rosangela Zacarias [3, 1]
Número total de Autores: 4
Afiliação do(s) autor(es):
[1] Univ Estadual Paulista, UNESP, Jaboticabal, SP - Brazil
[2] Univ Estadual Maranhao, Sao Luis, MA - Brazil
[3] Univ Estadual Paulista, Lab Imunoparasitol, Dept Patol Vet, Fac Ciencias Agr & Vet Julio Mesquita Filho, BR-14884900 Sao Paulo - Brazil
Número total de Afiliações: 3
Tipo de documento: Artigo Científico
Fonte: Parasitology Research; v. 109, n. 4, p. 1189-1192, OCT 2011.
Citações Web of Science: 11
Resumo

Few molecular studies have been done concerning the molecular characterization of Hepatozoon species among domestic and wild felids. The present work aimed to characterize molecularly the presence of Hepatozoon sp. DNA in cat blood samples from Sao Luis Island, Maranhao state, Northeastern Brazil. EDTA-whole blood samples were collected from 200 domestic cats with outdoor and wood areas access from Sao Luis, Maranhao, Brazil. Each sample of extracted DNA was used as a template in PCR reactions aiming to amplify a partial sequence of 18S rRNA of Hepatozoon spp. We also performed sequence alignment to establish the identity of the parasite species infecting these animals using DNA sequences based on 18S rRNA. From 200 sampled cats, Hepatozoon DNA was only found in one animal (0.5%). The found Hepatozoon DNA showed 97% of identity with Hemobartonella felis isolates 1 and 2 from Spain. When analyzing the phylogenetic tree, the found Hepatozoon DNA was in the same clade than H. felis isolates. Our findings suggest that more than one species of Hepatozoon could infect felids in Brazil. (AU)

Processo FAPESP: 09/53610-5 - Detecção e caracterização molecular de Hepatozoon SP. em felinos domésticos (Felis catus) de São Luís, Maranhão
Beneficiário:Caroline Placidi de Bortoli
Linha de fomento: Bolsas no Brasil - Iniciação Científica