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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Serotonergic neurons in the nucleus raphe obscurus contribute to interaction between central and peripheral ventilatory responses to hypercapnia

Texto completo
Autor(es):
da Silva, Glauber S. F. [1] ; Giusti, Humberto [1] ; Benedetti, Mauricio [1] ; Dias, Mirela B. [2] ; Gargaglioni, Luciane H. [3] ; Branco, Luiz Guilherme S. [4] ; Glass, Mogens L. [1]
Número total de Autores: 7
Afiliação do(s) autor(es):
[1] Univ Sao Paulo USP FMRP, Dept Physiol, BR-14049900 Ribeirao Preto, SP - Brazil
[2] Univ Fed Goias, Inst Biol Sci, Dept Physiol Sci, Goiania, Go - Brazil
[3] Sao Paulo State Univ UNESP FCAV Jaboticabal, Dept Anim Morphol & Physiol, Jaboticabal, SP - Brazil
[4] Univ Sao Paulo USP FORP, Dept Morphol Stomatol & Physiol, Ribeirao Preto, SP - Brazil
Número total de Afiliações: 4
Tipo de documento: Artigo Científico
Fonte: PFLUGERS ARCHIV-EUROPEAN JOURNAL OF PHYSIOLOGY; v. 462, n. 3, p. 407-418, SEP 2011.
Citações Web of Science: 31
Resumo

Serotonergic (5-HT) neurons in the nucleus raphe obscurus (ROb) are involved in the respiratory control network. However, it is not known whether ROb 5-HT neurons play a role in the functional interdependence between central and peripheral chemoreceptors. Therefore, we investigated the role of ROb 5-HT neurons in the ventilatory responses to CO(2) and their putative involvement in the central-peripheral CO(2) chemoreceptor interaction in unanaesthetised rats. We used a chemical lesion specific for 5-HT neurons (anti-SERT-SAP) of the ROb in animals with the carotid body (CB) intact or removed (CBR). Pulmonary ventilation (V (E)), body temperature and the arterial blood gases were measured before, during and after a hypercapnic challenge (7% CO(2)). The lesion of ROb 5-HT neurons alone (CB intact) or the lesion of 5-HT neurons of ROb+CBR did not affect baseline V (E) during normocapnic condition. Killing ROb 5-HT neurons (CB intact) significantly decreased the ventilatory response to hypercapnia (p < 0.05). The reduction in CO(2) sensitivity was approximately 15%. When ROb 5-HT neurons lesion was combined with CBR (anti-SERT-SAP+CBR), the V (E) response to hypercapnia was further decreased (-31.2%) compared to the control group. The attenuation of CO(2) sensitivity was approximately 30%, and it was more pronounced than the sum of the individual effects of central (ROb lesion; -12.3%) or peripheral (CBR; -5.5%) treatments. Our data indicate that ROb 5-HT neurons play an important role in the CO(2) drive to breathing and may act as an important element in the central-peripheral chemoreception interaction to CO(2) responsiveness. (AU)

Processo FAPESP: 07/51581-2 - Papel do núcleo obscuro da rafe na quimiossensibilidade ao CO2/pH
Beneficiário:Mogens Lesner Glass
Linha de fomento: Auxílio à Pesquisa - Regular
Processo FAPESP: 06/60696-5 - Papel do núcleo obscuro da rafe na quimiossensibilidade ao CO2/pH
Beneficiário:Glauber dos Santos Ferreira da Silva
Linha de fomento: Bolsas no Brasil - Doutorado