Complement system component 3 deficiency modulates the phenotypic profile of murine macrophages

Autor(es)

da Silva, Tiago Francisco ; Amamura, Thais Akemi ; Valadao, Iuri Cordeiro ; Carneiro, Milena Carvalho ; Freitas, Vanessa Morais ; Lepique, Ana Paula ; Isaac, Lourdes

• Tipo de documento: Artigo Científico
• Fonte: Cellular Immunology; v. 405, p. 10-pg., 2024-10-28.

Resumo

The Complement System is composed of more than 40 proteins that act in innate and adaptive immunity. C3 is the most abundant one and C3-deficient patients are more susceptible to recurrent and severe infections. Several studies have demonstrated the importance of C3 in controlling infections. However, its role in leukocyte biology is still poorly understood. This study aimed to evaluate several cellular parameters in macrophages from C3deficient mice and compare them to similar cells from wild-type counterparts. We observed that in the absence of C3, the population of F4/80low macrophages in the peritoneal cavity of thioglycolate-treated mice is diminished, probably due to the lack of chemotactic factors like C3a and low levels of C5a. Using fluorescence microscopy analysis, we observed that macrophages from C3-deficient mice exhibited morphological alterations when compared to similar cells from wild-type mice. We observed a significant increase in the expression of CD11c, which is part of CR4 (CD11c/CD18), in macrophages from C3-deficient compared to cells from wild-type mice. Treatment with 12-o-tetradecanoylphorbol-13-acetate, stimulated ROS production and MAPK activation by macrophages. However, these parameters were lower in macrophages from C3-deficient mice when compared to wild-type counterparts. In addition, the phagocytosis of iC3b-opsonized Zymosan particles was diminished in macrophages from C3-deficient mice. Our results suggest that C3 deficiency in C57Black/6 mice may influence specific morphological and functional parameters of macrophages, cells of fundamental importance for both the innate and acquired immune responses. (AU)

• Processo FAPESP: 19/01435-7 - Avaliação da fagocitose de leptospira por macrófagos peritoneais de camundongos C3 deficientes
• Beneficiário: Tiago Francisco da Silva
• Modalidade de apoio: Bolsas no Brasil - Mestrado
• Processo FAPESP: 17/12924-3 - Etiopatogênese da Leptospirose: contribuição do sistema complemento in vivo e in vitro para o controle da infecção e desencadeamento da resposta inflamatória tecidual: estudo de polimorfismos de genes do sistema complemento em pacientes com Leptospirose
• Beneficiário: Lourdes Isaac
• Modalidade de apoio: Auxílio à Pesquisa - Temático