| Texto completo | |
| Autor(es): |
Franca, Yasmin
;
Medeiros, Roberta Salzone
;
Viana, Ellen
;
de Azevedo, Lais Sampaio
;
Guiducci, Raquel
;
da Costa, Antonio Charlys
;
Luchs, Adriana
Número total de Autores: 7
|
| Tipo de documento: | Artigo Científico |
| Fonte: | FUNCTIONAL & INTEGRATIVE GENOMICS; v. 24, n. 3, p. 40-pg., 2024-06-01. |
| Resumo | |
In the early 2000s, the global emergence of rotavirus (RVA) G12P[8] genotype was noted, while G12P[6] and G12P[9] combinations remained rare in humans. This study aimed to characterize and phylogenetically analyze three Brazilian G12P[9] and four G12P[6] RVA strains from 2011 to 2020, through RT-PCR and sequencing, in order to enhance our understanding of the genetic relationship between human and animal-origin RVA strains. G12P[6] strains displayed a DS-1-like backbone, showing a distinct genetic clustering. G12P[6] IAL-R52/2020, IAL-R95/2020 and IAL-R465/2019 strains clustered with 2019 Northeastern G12P[6] Brazilian strains and a 2018 Benin strain, whereas IAL-R86/2011 strain grouped with 2010 Northern G12P[6] Brazilian strains and G2P[4] strains from the United States and Belgium. These findings suggest an African genetic ancestry and reassortments with co-circulating American strains sharing the same DS-1-like constellation. No recent zoonotic reassortment was observed, and the DS-1-like constellation detected in Brazilian G12P[6] strains does not seem to be genetically linked to globally reported intergenogroup G1/G3/G9/G8P[8] DS-1-like human strains. G12P[9] strains exhibited an AU-1-like backbone with two different genotype-lineage constellations: IAL-R566/2011 and IAL-R1151/2012 belonged to a VP3/M3.V Lineage, and IAL-R870/2013 to a VP3/M3.II Lineage, suggesting two co-circulating strains in Brazil. This genetic diversity is not observed elsewhere, and the VP3/M3.II Lineage in G12P[9] strains seems to be exclusive to Brazil, indicating its evolution within the country. All three G12P[9] AU-1-like strains were closely relate to G12P[9] strains from Paraguay (2006-2007) and Brazil (2010). Phylogenetic analysis also highlighted that all South American G12P[9] AU-1-like strains had a common origin and supports the hypothesis of their importation from Asia, with no recent introduction from globally circulating G12P[9] strains or reassortments with local G12 strains P[8] or P[6]. Notably, certain genes in the Brazilian G12P[9] AU-1-like strains share ancestry with feline/canine RVAs (VP3/M3.II, NSP4/E3.IV and NSP2/N3.II), whereas NSP1/A3.VI likely originated from artiodactyls, suggesting a history of zoonotic transmission with human strains. This genomic data adds understanding to the molecular epidemiology of G12P[6] and G12P[9] RVA strains in Brazil, offering insights into their genetic diversity and evolution. (AU) | |
| Processo FAPESP: | 20/11182-6 - Epidemiologia molecular de cepas de rotavirus g8 isoladas no brasil associadas aos genótipos p[4], p[6] ou p[8] |
| Beneficiário: | Roberta Salzone Medeiros |
| Modalidade de apoio: | Bolsas no Brasil - Iniciação Científica |
| Processo FAPESP: | 17/50333-7 - Plano de desenvolvimento institucional em pesquisa do Instituto Adolfo Lutz (PDIp) |
| Beneficiário: | Carlos Henrique Camargo |
| Modalidade de apoio: | Auxílio à Pesquisa - Programa Modernização de Institutos Estaduais de Pesquisa |
| Processo FAPESP: | 20/02469-0 - Constelação completa dos genótipos de cinco cepas raras de rotavirus g12p[9] detectadas no brasil em 2011-2013 |
| Beneficiário: | Yasmin França Viana Pires de Souza |
| Modalidade de apoio: | Bolsas no Brasil - Iniciação Científica |
| Processo FAPESP: | 20/14786-0 - Vigilância e epidemiologia molecular dos Bocavirus humanos associados à gastroenterite aguda |
| Beneficiário: | Adriana Luchs |
| Modalidade de apoio: | Auxílio à Pesquisa - Regular |
| Processo FAPESP: | 22/02529-8 - Epidemiologia molecular dos bocavírus humanos em amostras fecais históricas no brasil (1998-2005) |
| Beneficiário: | Ellen Viana de Souza |
| Modalidade de apoio: | Bolsas no Brasil - Mestrado |
| Processo FAPESP: | 21/09064-8 - Vigilância e epidemiologia molecular dos Bocavírus humanos associados à gastroenterite aguda |
| Beneficiário: | Yasmin França Viana Pires de Souza |
| Modalidade de apoio: | Bolsas no Brasil - Programa Capacitação - Treinamento Técnico |
| Processo FAPESP: | 15/12944-9 - Investigando a evolução de cepas animais de rotavírus infectando humanos |
| Beneficiário: | Adriana Luchs |
| Modalidade de apoio: | Auxílio à Pesquisa - Regular |