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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Optimized Enzymatic Synthesis of Hesperidin Fatty Acid Esters in a Two-Phase System Containing Ionic Liquid

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Autor(es):
Melo Branco de Araujo, Maria Elisa [1] ; Contesini, Fabiano Jares [2] ; Moreira Franco, Yollanda Edwirges [1] ; Frankland Sawaya, Alexandra C. H. [3] ; Alberto, Thiago Grando [1] ; Dalfre, Natalia [1] ; Carvalho, Patricia de Oliveira [1]
Número total de Autores: 7
Afiliação do(s) autor(es):
[1] Univ Sao Francisco, BR-12916900 Braganca Paulista, SP - Brazil
[2] State Univ Campinas UNICAMP, Fac Food Engn, Dept Food Sci, Lab Food Biochem, BR-13083862 Campinas, SP - Brazil
[3] State Univ Campinas UNICAMP, Inst Biol, Dept Plant Biol, BR-13083970 Campinas, SP - Brazil
Número total de Afiliações: 3
Tipo de documento: Artigo Científico
Fonte: Molecules; v. 16, n. 8, p. 7171-7182, AUG 2011.
Citações Web of Science: 11
Resumo

Response surface methodology (RSM) based on a five-level, three-variable central composite design (CCD) was employed for modeling and optimizing the conversion yield of the enzymatic acylation of hesperidin with decanoic acid using immobilized Candida antarctica lipase B (CALB) in a two-phase system containing {[}bmim]BF(4). The three variables studied (molar ratio of hesperidin to decanoic acid, {[}bmim]BF(4)/acetone ratio and lipase concentration) significantly affected the conversion yield of acylated hesperidin derivative. Verification experiments confirmed the validity of the predicted model. The lipase showed higher conversion degree in a two-phase system using {[}bmim] BF4 and acetone compared to that in pure acetone. Under the optimal reaction conditions carried out in a single-step biocatalytic process when the water content was kept lower than 200 ppm, the maximum acylation yield was 53.6%. (AU)

Processo FAPESP: 09/09224-3 - Biotransformação de polifenóis e potencial antioxidante
Beneficiário:Patrícia de Oliveira Carvalho
Linha de fomento: Auxílio à Pesquisa - Regular