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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

The role of the TP53 gene during rat tongue carcinogenesis induced by 4-nitroquinoline 1-oxide

Texto completo
Autor(es):
Minicucci, E. M. [1] ; Ribeiro, D. A. [2] ; da Silva, G. N. [3] ; Pardini, M. I. M. C. [4] ; Montovani, J. C. [5] ; Salvadori, D. M. F. [3]
Número total de Autores: 6
Afiliação do(s) autor(es):
[1] Sao Paulo State Univ, UNESP, Dept Dermatol & Radiotherapy, Sao Paulo, SP - Brazil
[2] Univ Fed Sao Paulo, UNIFESP, Dept Biosci, BR-11060001 Santos, SP - Brazil
[3] Sao Paulo State Univ, UNESP, Dept Pathol, Sao Paulo, SP - Brazil
[4] Sao Paulo State Univ, UNESP, Dept Clin Med, Sao Paulo, SP - Brazil
[5] Sao Paulo State Univ, UNESP, Dept Ophthalmol & Otorhinolaryngol, Sao Paulo, SP - Brazil
Número total de Afiliações: 5
Tipo de documento: Artigo Científico
Fonte: EXPERIMENTAL AND TOXICOLOGIC PATHOLOGY; v. 63, n. 5, p. 483-489, JUL 2011.
Citações Web of Science: 11
Resumo

The medium-term tongue carcinogenesis assay is a useful model for studying oral squamous cell carcinomas phase by phase. The aim of the present study was to investigate the expression of p53 by immunohistochemistry and examine the DNA sequence of exons 5, 6, 7, and 8 of Tp53 for mutations during rat tongue carcinogenesis induced by 4-nitroquinoline 1-oxide (4NQO). A total of 30 male Wistar rats were treated with 4-nitroquinoline 1-oxide in their drinking water for 4, 12, and 20 weeks at a dose of 50 ppm. Ten animals were used as negative controls. No histopathological changes in the tongue epithelia were observed in the control group or in the treatment group after 4 weeks of 4NQO. Following 12 weeks of treatment, hyperplasia as well as epithelial dysplasia was found in both mild and moderate forms. At 20 weeks, moderate and/or severe oral dysplasia and squamous cell carcinoma of the tongue were found, and the majority of animals had squamous cell carcinoma. The levels of p53 protein were increased (p < 0.05) in pre-neoplastic lesions and in squamous cell carcinomas in some of the tumor cells in squamous cell carcinomas. No mutations were found in any of the exons that were evaluated after the 4-, 12-, or 20-week treatments. Taken together, our results suggest that p53 expression may be an important event in the malignant conversion, whereas Tp53 mutations are not involved in the multi-step tongue carcinogenesis of Wistar rats induced by 4NQO. (C) 2010 Elsevier GmbH. All rights reserved. (AU)

Processo FAPESP: 05/59156-3 - Marcadores moleculares da carcinogênese bucal: estudo comparativo em dois sítios anatômicos
Beneficiário:Daisy Maria Favero Salvadori
Linha de fomento: Auxílio à Pesquisa - Regular
Processo FAPESP: 07/01228-4 - Carcinogênese bucal quimicamente induzida pela 4-nitroquinolina 1-óxido em ratos: possíveis biomarcadores envolvidos em sua patogênese
Beneficiário:Daniel Araki Ribeiro
Linha de fomento: Auxílio à Pesquisa - Apoio a Jovens Pesquisadores