| Texto completo | |
| Autor(es): Mostrar menos - |
de Sa, Jessica Marostica
;
Thongpan, Ilada
;
Busso, Jefferson de Souza
;
Rodrigues, Thaina dos Santos
;
Chen, Phylip
;
Helena, Alvaro Luiz
;
Regasini, Luis Octavio
;
Fossey, Marcelo Andres
;
Caruso, icaro Putinhon
;
de Souza, Fatima Pereira
;
Peeples, Mark Edward
Número total de Autores: 11
|
| Tipo de documento: | Artigo Científico |
| Fonte: | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES; v. 25, n. 24, p. 19-pg., 2024-12-01. |
| Resumo | |
Respiratory syncytial virus (RSV) is one of the most prevalent viruses that causes severe acute lower respiratory tract infections (ALRTIs) in the elderly and young children. There is no specific drug to treat RSV, only a broad-spectrum antiviral, ribavirin, which is only used in critical cases. Our research group is investigating antiviral agents of natural origin, such as coumarins and flavonoids, that may help reduce or prevent RSV infection. The cytotoxic concentrations of coumarins and hesperetin were tested on A549 and HEp-2 cells and used in inhibition tests in which 80% of the cells were viable. The anti-RSV action of the molecules was analyzed in A549 and HEp-2 cells and in HBE cell cultures infected with RSV-luc or rgRSV. We also encapsulated the compounds using beta-cyclodextrin to improve the permeability and solubility of the molecules. Esculetin and 4-methyl inhibited rgRSV effectively on A549 and HEp-2 cells after 24 hpi, and when they were encapsulated, coumarin, esculetin, and hesperetin presented inhibition against rgRSV in HBE culture. The coumarins inhibit RSV replication in cell culture and even manage to overcome the mucus barriers of the HBE cultures, and beta-cyclodextrin was essential for some of the coumarins to enter the cell and therefore to reach their targets. (AU) | |
| Processo FAPESP: | 22/10481-5 - Compostos naturais como candidatos antivirais contra vírus sincicial respiratório humano (hRSV) |
| Beneficiário: | Jéssica Maróstica de Sá |
| Modalidade de apoio: | Bolsas no Exterior - Estágio de Pesquisa - Doutorado |
| Processo FAPESP: | 09/53989-4 - EMU: aquisição de espectrômetro de ressonância magnética nuclear para estudos de biomoléculas |
| Beneficiário: | Raghuvir Krishnaswamy Arni |
| Modalidade de apoio: | Auxílio à Pesquisa - Programa Equipamentos Multiusuários |
| Processo FAPESP: | 20/13582-1 - Cumarinas e hesperetinas modificadas como candidatas antivirais ao bolso hidrofóbico no sítio de interação nucleoproteína/fosfoproteína do Vírus Sincicial Respiratório Humano |
| Beneficiário: | Jéssica Maróstica de Sá |
| Modalidade de apoio: | Bolsas no Brasil - Doutorado |
| Processo FAPESP: | 21/14349-1 - Avaliação do potencial efeito dos derivados de Kaempferol e chalconas sobre o ciclo de replicação do RSV em cultivo celular: uma investigação dos mediadores proteicos e metabólicos no ciclo viral |
| Beneficiário: | Fátima Pereira de Souza |
| Modalidade de apoio: | Auxílio à Pesquisa - Regular |