Diversity and quantitative analysis of Archaea in aggressive periodontitis and periodontally healthy subjects

Autor(es)

Matarazzo, Flavia ^[1] ; Ribeiro, Adriana Costa ^[1] ; Feres, Magda ^[2] ; Faveri, Marcelo ^{[1, 2]} ; Alves Mayer, Marcia Pinto ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Inst Ciencias Biomed, Dept Microbiol, BR-05508900 Sao Paulo - Brazil

^[2] Univ Guarulhos, Dent Res Div, Dept Periodontol, Guarulhos, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: JOURNAL OF CLINICAL PERIODONTOLOGY; v. 38, n. 7, p. 621-627, JUL 2011.
• Citações Web of Science: 42

Resumo

P>Aim To investigate the diversity, levels and proportions of Archaea in the subgingival biofilm of generalized aggressive periodontitis (GAgP; n=30) and periodontally healthy (PH; n=30) subjects. Materials and methods Diversity was determined by sequencing archaeal 16S rRNA gene libraries from 20 samples (10/group). The levels and proportions of Archaea were analysed by quantitative PCR (qPCR) in four and two samples/subject in GAgP and PH groups, respectively. Results Archaea were detected in 27/28 subjects and 68% of the sites of the GAgP group, and in 26/30 subjects and 58.3% sites of the PH group. Methanobrevibacter oralis was found in all 20 samples studied, Methanobacterium curvum/congolense in three GAgP and six PH samples, and Methanosarcina mazeii in four samples from each group. The levels and proportions of Archaea were higher in GAgP than in PH, whereas no differences were observed between the two probing depth category sites from the GAgP group. Conclusion Archaea were frequently found in subjects with periodontal health and GAgP, especially M. oralis. However, the higher levels and proportions (Archaea/total prokaryotes) of this domain observed in GAgP in comparison with PH subjects indicate a possible role of some of these microorganisms as an environmental modifier in GAgP. (AU)

• Processo FAPESP: 06/52890-6 - Identificação de possíveis organismos associados à periodontite agressiva por análise clonal de 16S rRNA
• Beneficiário: Marcia Pinto Alves Mayer
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 07/56413-0 - Efeitos clínicos e microbiológicos da associação sistêmica de amoxicilina e metronidazol à raspagem e alisamento radicular em indivíduos com doença periodontal agressiva generalizada
• Beneficiário: Marcelo de Faveri
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 07/56509-8 - Diversidade e quantificacao de organismos do dominio archaea em amostras de biofilme subgengival de individuos com periodontite agressiva e saude periodontal.
• Beneficiário: Flávia Matarazzo
• Modalidade de apoio: Bolsas no Brasil - Doutorado