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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Chemical and biological characterization of four new linear cationic alpha-helical peptides from the venoms of two solitary eumenine wasps

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Autor(es):
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Rangel, Marisa [1] ; dos Santos Cabrera, Marcia Perez [2] ; Kazuma, Kohei [3] ; Ando, Kenji [3] ; Wang, Xiaoyu [3] ; Kato, Manabu [4] ; Nihei, Ken-ichi [5] ; Hirata, Izaura Yoshico [6] ; Cross, Tyra J. [7] ; Garcia, Angelica Nunes [1] ; Faquim-Mauro, Eliana L. [1] ; Franzolin, Marcia Regina [8] ; Fuchino, Hiroyuki [9] ; Mori-Yasumoto, Kanami [10] ; Sekita, Setsuko [10] ; Kadowaki, Makoto [3] ; Satake, Motoyoshi [3] ; Konno, Katsuhiro [3]
Número total de Autores: 18
Afiliação do(s) autor(es):
[1] Butantan Inst, Immunopathol Lab, BR-05503900 Sao Paulo - Brazil
[2] Sao Paulo State Univ, IBILCE, Dept Phys, BR-15054000 Sao Jose Do Rio Preto, SP - Brazil
[3] Toyama Univ, Inst Nat Med, Toyama 9300194 - Japan
[4] Yamada Apiculture Ctr Inc, Okayama 7080393 - Japan
[5] Utsunomiya Univ, Fac Agr, Utsunomiya, Tochigi 3218505 - Japan
[6] Univ Fed Sao Paulo, Dept Biophys, Paulista Med Sch, BR-04044020 Sao Paulo - Brazil
[7] Univ Victoria, Genome BC Prote Ctr, Victoria, BC V8Z 7X8 - Canada
[8] Butantan Inst, Bacteriol Lab, BR-05503900 Sao Paulo - Brazil
[9] Natl Inst Biomed Innovat, Res Ctr Med Plant Resources, Tsukuba, Ibaraki 3050843 - Japan
[10] Tokushima Bunri Univ, Fac Pharmaceut Sci, Kagawa 7692193 - Japan
Número total de Afiliações: 10
Tipo de documento: Artigo Científico
Fonte: Toxicon; v. 57, n. 7-8, p. 1081-1092, JUN 2011.
Citações Web of Science: 12
Resumo

Four novel peptides were isolated from the venoms of the solitary eumenine wasps Eumenes rubrofemoratus and Eumenes fraterculus. Their sequences were determined by MALDI-TOF/TOF (matrix assisted laser desorption/ionization time-of-flight mass spectrometry) analysis, Edman degradation and solid-phase synthesis. Two of them, eumenitin-R (LNLKGLIKKVASLLN) and eumenitin-F (LNLKGLFKKVASLLT), are highly homologous to eumenitin, an antimicrobial peptide from a solitary eumenine wasp, whereas the other two, EMP-ER (FDIMGLIKKVAGAL-NH(2)) and EMP-EF (FDVMGIIKKIAGAL-NH(2)), are similar to eumenine mastoparan-AF (EMP-AF), a mast cell degranulating peptide from a solitary eumenine wasp. These sequences have the characteristic features of linear cationic cytolytic peptides; rich in hydrophobic and basic amino acids with no disulfide bond, and accordingly, they can be predicted to adopt an amphipathic alpha-helix secondary structure. In fact, the CD (circular dichroism) spectra of these peptides showed significant alpha-helical conformation content in the presence of TFE (trifluoroethanol), SDS (sodium dodecylsulfate) and asolectin vesicles. In the biological evaluation, all the peptides exhibited a significant broad-spectrum antimicrobial activity, and moderate mast cell degranulation and leishmanicidal activities, but showed virtually no hemolytic activity. (C) 2011 Elsevier Ltd. All rights reserved. (AU)

Processo FAPESP: 08/00173-4 - Prospecção de peptídeos naturais antimicrobianos formadores de poros em membranas celulares e bicamadas lipídicas artificiais
Beneficiário:Marisa Rangel
Linha de fomento: Auxílio à Pesquisa - Regular