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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

MicroRNAs 29 are involved in the improvement of ventricular compliance promoted by aerobic exercise training in rats

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Autor(es):
Soci, U. P. R. [1] ; Fernandes, T. [1] ; Hashimoto, N. Y. [1] ; Mota, G. F. [1] ; Amadeu, M. A. [1] ; Rosa, K. T. [2] ; Irigoyen, M. C. [2] ; Phillips, M. I. [3] ; Oliveira, E. M. [1]
Número total de Autores: 9
Afiliação do(s) autor(es):
[1] Univ Sao Paulo, Sch Phys Educ & Sport, Lab Biochem Motor Act, BR-05508900 Sao Paulo - Brazil
[2] Univ Sao Paulo, Hypertens Unit, Heart Inst InCor, Sch Med, BR-05508900 Sao Paulo - Brazil
[3] Keck Grad Inst Appl Life Sci, Lab Stem Cells, Claremont, CA - USA
Número total de Afiliações: 3
Tipo de documento: Artigo Científico
Fonte: Physiological Genomics; v. 43, n. 11, p. 665-673, JUN 2011.
Citações Web of Science: 92
Resumo

Soci UPR, Fernandes T, Hashimoto NY, Mota GF, Amadeu MA, Rosa KT, Irigoyen MC, Phillips MI, Oliveira EM. MicroRNAs 29 are involved in the improvement of ventricular compliance promoted by aerobic exercise training in rats. Physiol Genomics 43: 665-673, 2011. First published March 29, 2011; doi:10.1152/physiolgenomics.00145.2010.-MiRNAs regulate cardiac development, hypertrophy, and angiogenesis, but their role in cardiac hypertrophy (CH) induced by aerobic training has not previously been studied. Aerobic training promotes physiological CH preserving cardiac function. This study assessed involvement of miRNAs-29 in CH of trained rats. Female Wistar rats (n = 7/group) were randomized into three groups: sedentary (S), training 1 (T1), training 2 (T2). T1: swimming sessions of 60 min/5 days/wk/10 wk. T2: similar to T1 until 8th wk. On the 9th wk rats swam 2x/day, and on the 10th wk 3x/day. MiRNAs analysis was performed by miRNA microarray and confirmed by real-time PCR. We assessed: markers of training, CH by ratio of left ventricle (LV) weight/body wt and cardiomyocytes diameter, pathological markers of CH (ANF, skeletal alpha-actin, alpha/beta-MHC), collagen I and III (COLIAI and COLIIIAI) by real-time PCR, protein collagen by hydroxyproline (OH-proline) concentration, CF and CH by echocardiography. Training improved aerobic capacity and induced CH. MiRNAs-1, 133a, and 133b were downregulated as observed in pathological CH, however, without pathological markers. MiRNA-29c expression increased in T1 (52%) and T2 (123%), correlated with a decrease in COLIAI and COLIIIAI expression in T1 (27%, 38%) and T2 (33%, 48%), respectively. MiRNA-29c was inversely correlated to OH-proline concentration (r = 0.61, P = 0.05). The E/A ratio increased in T2, indicating improved LV compliance. Thus, these results show that aerobic training increase miR-29 expression and decreased collagen gene expression and concentration in the heart, which is relevant to the improved LV compliance and beneficial cardiac effects, associated with aerobic high performance training. (AU)

Processo FAPESP: 07/56771-4 - Efeitos do treinamento físico aeróbico na modulação das células progenitoras endoteliais derivadas da medula óssea em ratos espontaneamente hipertensos (SHR)
Beneficiário:Tiago Fernandes
Linha de fomento: Bolsas no Brasil - Mestrado