| Texto completo | |
| Autor(es): |
Moreira, Natalia Columbaro
;
Abichabki, Nathalia
;
Ferreira, Joseane Cristina
;
Martinez, Roberto
;
Darini, Ana Lucia da Costa
;
Andrade, Leonardo Neves
Número total de Autores: 6
|
| Tipo de documento: | Artigo Científico |
| Fonte: | JOURNAL OF GLOBAL ANTIMICROBIAL RESISTANCE; v. 42, p. 7-pg., 2025-05-25. |
| Resumo | |
Antimicrobial resistance is a major health care problem as well as a concern for global public health. As a result, the use of nonantibiotic antimicrobials, such as heavy metals and biocides, has increased in a bid to control the spread of antibiotic-resistant bacteria. Consequently, heavy metal tolerance genes (HMTGs) and biocide tolerance genes (BTGs) have been more frequently detected in Gram-negative bacilli. In this study, we searched for acquired HMTGs, BTGs, and antibiotic resistance genes (ARGs) and determined the MICs of common heavy metals and biocides in multidrug-resistant and susceptible Gram-negative bacilli clinical isolates. A high frequency of silA and pcoD genes was mainly detected among Klebsiella spp. and Enterobacter cloacae regardless of their susceptible profile. The merA gene was also found in isolates carrying silA/pcoD genes. ARGs were detected in isolates that harboured silA and/or pcoD genes. BTGs (qacAE, ydgE, ydgF, mdfA, and emrE) were mostly detected in Klebsiella pneumoniae and E. cloacae isolates regardless of their susceptibility profile, and these isolates often co-harboured HMTGs and/or ARGs. Higher copper sulphate MIC values were obtained under aerobic conditions, regardless of the presence or absence of pcoD and/or silA genes. Nevertheless, in most isolates carrying pcoD/silA, higher copper sulphate MIC values were determined under anaerobic conditions. Regarding AgNO3, no significant differences in MIC values were observed for isolates with or without the silA gene. Our results show a broad distribution of HMTGs, BTGs, and ARGs in bacteria causing health care-associated infections, which could contribute to the co-selection of hospital pathogens resistant to multiple and diverse antimicrobials. (c) 2025 The Authors. Published by Elsevier Ltd on behalf of International Society for Antimicrobial Chemotherapy. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/) (AU) | |
| Processo FAPESP: | 18/24286-4 - Investigação fenotípica e molecular da resistência a antibióticos e tolerância a biocidas e metais pesados em bacilos gram-negativos isolados em hospital universitário |
| Beneficiário: | Natália Columbaro Moreira |
| Modalidade de apoio: | Bolsas no Brasil - Doutorado Direto |