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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Analysis of genotypic variation in genes associated with virulence in Aggregatibacter actinomycetemcomitans clinical isolates

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Autor(es):
Pinheiro, E. T. [1] ; Kawamoto, D. [1] ; Ota-Tsuzuki, C. [1] ; Almeida, L. R. S. [1] ; Nunes, A. C. R. [1] ; Longo, P. L. [1] ; Wikstrom, M. [2] ; Mayer, M. P. A. [1]
Número total de Autores: 8
Afiliação do(s) autor(es):
[1] Univ Sao Paulo, Inst Biomed Sci, Dept Microbiol, BR-05508900 Sao Paulo - Brazil
[2] Univ Gothenburg, Sch Dent, Dept Microbiol, Gothenburg - Sweden
Número total de Afiliações: 2
Tipo de documento: Artigo Científico
Fonte: JOURNAL OF PERIODONTAL RESEARCH; v. 46, n. 3, p. 310-317, JUN 2011.
Citações Web of Science: 5
Resumo

Background and Objective: Although certain serotypes of Aggregatibacter actinomycetemcomitans are associated more with aggressive periodontitis than are other serotypes, the correlation between distinct lineages and virulence traits in this species is poorly understood. This study aimed to evaluate the polymorphism of genes encoding putative virulence factors of clinical isolates, and to correlate these findings with A. actinomycetemcomitans serotypes, genotypes and periodontal status of the hosts. Material and Methods: Twenty-six clinical isolates from diverse geographic populations with different periodontal conditions were evaluated. Genotyping was performed using pulse-field gel electrophoresis. Polymorphisms in the genes encoding leukotoxin, Aae, ApaH and determinants for serotype-specific O polysaccharide were investigated. Results: The isolates were classified into serotypes a-f, and exhibited three apaH genotypes, five aae alleles and 25 macrorestriction profiles. Two serotype b isolates (7.7%), obtained from Brazilian patients with aggressive periodontitis, were associated with the highly leukotoxic genotype; these isolates showed identical fingerprint patterns and aae and apaH genotypes. Serotype c, obtained from various periodontal conditions, was the most prevalent among Brazilian isolates, and isolates were distributed in two aae alleles, but formed a genetically distinct group based on apaH analysis. Cluster analysis showed a close relationship between fingerprinting genotypes and serotypes/apaH genotypes, but not with aae genotypes. Conclusion: Apart from the deletion in the ltx promoter region, no disease-associated markers were identified. Non-JP2-like strains recovered from individuals with periodontal disease exhibited considerable genetic variation regarding aae/apaH genotypes, serotypes and XhoI DNA fingerprints. (AU)

Processo FAPESP: 03/08598-0 - Fatores de virulência de A. actinomycetemcomitans: participação na doença, regulação da expressão, diversidade e resposta imune
Beneficiário:Marcia Pinto Alves Mayer
Linha de fomento: Auxílio à Pesquisa - Temático
Processo FAPESP: 06/55512-2 - Tipagem de cepas de Actinobacillus actinomycetemcomitans isoladas de pacientes com doença periodontal por PFGE e sua relação com os fatores de virulência
Beneficiário:Ericka Tavares Pinheiro
Linha de fomento: Bolsas no Brasil - Pós-Doutorado