| Texto completo | |
| Autor(es): Mostrar menos - |
Roque-Borda, Cesar Augusto
;
Chavez-Moran, Marco Roberto
;
Primo, Laura Maria Duran Gleriani
;
Montesinos, Jose C. E. Marquez
;
Cardoso, Vinicius Martinho Borges
;
Saraiva, Mauro M. S.
;
Marcos, Caroline Maria
;
Chorilli, Marlus
;
Albericio, Fernando
;
de la Torreh, Beatriz G.
;
Pavan, Fernando Rogerio
;
Meneguinc, Andreia Bagliotti
Número total de Autores: 12
|
| Tipo de documento: | Artigo Científico |
| Fonte: | FOOD HYDROCOLLOIDS; v. 167, p. 16-pg., 2025-04-24. |
| Resumo | |
Integrating antimicrobial peptides (AMPs) into alginate-pectin-based microstructured delivery systems enhances the controlled release and stability of bioactive like resveratrol. This study examines spray-dried microparticles utilizing alginate and pectin as primary carbohydrate polymers combined with aggregated AMPs for dual antioxidant and antimicrobial effects. The AMP was self-assembled into nanostructures upon interaction with alginate and pectin, which were subsequently incorporated into spray-dried microparticles, resulting in a stable delivery system. In silico modeling and in vitro release studies under simulated gastrointestinal conditions showed that AMP-containing microparticles provided a slower, sustained resveratrol release than peptide-free systems. The Weibull model best described the release, indicating a multi-phase behavior driven by diffusion and erosion. In gastric simulated conditions, the alginate-pectin matrices with AMP improved structural integrity, reducing release, while in intestinal ones, partial erosion enabled controlled release. In vivo infection studies using Galleria mellonella demonstrated significant reductions in inflammation and bacterial load 48 h post-infection. These results suggest that alginate-pectin microparticles with aggregated AMPs enhance the antioxidant release and maintain antimicrobial activity, making them promising for gastrointestinal applications. According to these promising findings, the next step studies will aim to enhance site-specific therapeutic performance through formulation refinement and targeted delivery strategies. (AU) | |
| Processo FAPESP: | 21/14603-5 - Descoberta e desenho de fármacos: análogos do peptídeo antimicrobiano B1CTcu5 promissores contra o Mycobacterium tuberculosis |
| Beneficiário: | Cesar Augusto Roque Borda |
| Modalidade de apoio: | Bolsas no Exterior - Estágio de Pesquisa - Doutorado |
| Processo FAPESP: | 20/16573-3 - Estudos in vitro e in vivo de análogos do peptídeo antimicrobiano B1CTcu5 encapsulados em micropartículas colón-específicas frente ao Mycobacterium tuberculosis |
| Beneficiário: | Cesar Augusto Roque Borda |
| Modalidade de apoio: | Bolsas no Brasil - Doutorado |
| Processo FAPESP: | 23/01664-1 - Síntese e caracterização de análogos do peptídeo antimicrobiano "B1CTcu5" encapsulados em micropartículas colón-específicas e estudos in vitro e in vivo frente ao Mycobacterium tuberculosis |
| Beneficiário: | Fernando Rogério Pavan |
| Modalidade de apoio: | Auxílio à Pesquisa - Regular |