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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Antigenicity of Primary Endodontic Infection against Macrophages by the Levels of PGE(2) Production

Texto completo
Autor(es):
Martinho, Frederico C. [1] ; Maia Chiesa, Wanderson Miguel [1] ; Leite, Fabio R. M. [2] ; Cirelli, Joni A. [3] ; Gomes, Brenda P. F. A. [1]
Número total de Autores: 5
Afiliação do(s) autor(es):
[1] Univ Estadual Campinas, Piracicaba Dent Sch, Endodont Div, Dept Restorat Dent, Sao Paulo - Brazil
[2] Univ Fed Pelotas, Sch Dent, Periodont Div, Dept Semiol & Clin, Pelotas - Brazil
[3] State Univ Sao Paulo, Araraquara Dent Sch, Periodont Div, Dept Diag & Oral Surg, Sao Paulo - Brazil
Número total de Afiliações: 3
Tipo de documento: Artigo Científico
Fonte: JOURNAL OF ENDODONTICS; v. 37, n. 5, p. 602-607, MAY 2011.
Citações Web of Science: 15
Resumo

Introduction: Root canal contents are potent stimuli for proinflammatory cytokines involved in apical periodontitis. This study investigated target gram-negative bacterial species and endotoxins in primary endodontic infection with apical periodontitis, determined their antigenicity against macrophages through the levels of PGE(2), and evaluated their relationship with clinical findings. Methods: Samples were taken from 21 root canals with primary infection and apical periodontitis by using paper points. Polymerase chain reaction (165 rDNA) was used for bacterial detection and limulus amebocyte lysate assay for endotoxin measurement. Levels of prostaglandin E2 (PGE(2)) were measured by enzyme-linked immunosorbent assay (Duoset Kit; R\&D, Minneapolis, MN). Results: Prevotella nigrescens (13/21), Fusobacterium nucleatum (6/21), and Porphyromonas endodontalis (6/21) were the most frequently observed species. A positive association was found between F nucleatum and P endodontalis (P < .05). A correlation was found between the number of gram-negative bacterial species and the levels of endotoxins, as well as PGE2 (P < .05). Higher levels of endotoxin were detected in teeth with exudation, whereas elevated levels of PGE(2) were found in teeth with tenderness to percussion and pain on palpation. Conclusions: Our findings imply an additive effect between the number of gram-negative bacterial species involved in endodontic infection regarding the induction of proinflammatory cytokine by macrophage cells. Moreover, teeth with clinical symptomatology were related to higher levels of endotoxins and PGE2 secretion. (J Endod 2011;37:602-607) (AU)

Processo FAPESP: 07/58518-4 - Análise da microbiota de canais radiculares de dentes humanos infectados através da cultura, clonagem e sequenciamento do gene 16S rRNA
Beneficiário:Brenda Paula Figueiredo de Almeida Gomes
Linha de fomento: Auxílio à Pesquisa - Regular
Processo FAPESP: 08/57551-0 - Microbiota dos canais radiculares de dentes com insucesso do tratamento endodôntico e análise da suscetibilidade antimicrobiana e dos fatores de virulência dos Enterococcus faecalis isolados
Beneficiário:Brenda Paula Figueiredo de Almeida Gomes
Linha de fomento: Auxílio à Pesquisa - Regular
Processo FAPESP: 08/57954-8 - Estudo microbiológico, quantificação de endotoxinas e investigação da atividade endotóxica de diferentes LPS bacterianos isolados de canais radiculares de dentes com necrose pulpar e presença de lesão periapiacal
Beneficiário:Frederico Canato Martinho
Linha de fomento: Bolsas no Brasil - Doutorado
Processo FAPESP: 08/58299-3 - Estudo microbiológico e de endotoxinas em canais radiculares de dentes com necrose pulpar e lesão periapical e avaliação da atividade endotóxica de LPS isolados de canais radiculares infectados
Beneficiário:Brenda Paula Figueiredo de Almeida Gomes
Linha de fomento: Auxílio à Pesquisa - Regular