| Texto completo | |
| Autor(es): |
Sales, Luciana Solera
;
Hewitt, Benjamin
;
Muchova, Maria
;
Brightetnti, Fernanda Lourenca
;
Kuehne, Sarah A.
;
Grant, Melissa M.
;
Milward, Michael R.
Número total de Autores: 7
|
| Tipo de documento: | Artigo Científico |
| Fonte: | ARCHIVES OF ORAL BIOLOGY; v. 178, p. 12-pg., 2025-10-01. |
| Resumo | |
Objectives: This study aimed to evaluate the anti-inflammatory and antioxidant activity of morin in an epithelial cell culture inflammatory model, as well as its antimicrobial activity against periodontal pathogens. Effects of morin alone or slow-release from a polymer-based formulation were compared. Methods: An oral epithelial cell line (H400) was pre-conditioned with 0.125 mg/mL morin (free morin or morin in the formulation) for 8 h. The cells were challenged with heat-killed Fusobacterium nucleatum and Porphyromonas gingivalis (MOI 100:1) for 24 h. The production of GM-CSF was measured by enzyme-linked immunosorbent assay (ELISA) and the gene expression levels of IL-8, GM-CSF, IL1B, NF-kB and NLRP3 were evaluated by RT - PCR. Neutrophils were pre-conditioned with 0.01 mg/mL morin and stimulated immediately with heat-killed F. nucleatum or P. gingivalis (MOI 100:1) for 2.5 h and then total reactive-oxygen-species (ROS) levels were determined using enhanced chemiluminescence. F. nucleatum and P. gingivalis as well as a multi-species biofilm (Streptococcus oralis, F. nucleatum and P. gingivalis) were exposed to 1 mg/mL morin for 24 h, planktonic culture and for 7 days, multi-species biofilm. After exposure, the microbial viability (CFU/mL) and biofilm biomass (crystal violet assay) were determined. Live/dead staining and confocal laser scanning microscopy were also performed. Data were submitted to one way ANOVA followed by Tukey's post-hoc test (alpha=0.05). Results: Morin and the polymer-based formulation containing morin significantly attenuated secretion of GM-CSF, significantly reduced ROS generation and the gene expression levels of the cytokines tested (p < 0.05). Microbial viability and the biofilm biomass significantly reduced (p < 0.05) after treatment with morin and the polymer-based formulation containing morin. Conclusions: Morin showed an anti-inflammatory and antioxidant effect as well as antimicrobial properties against periodontal pathogens. In addition, polymer-based formulation enhanced these effects. (AU) | |
| Processo FAPESP: | 19/08375-0 - Potencialização do efeito anticárie e antibiofilme de substâncias convencionais: avaliação em modelo de biofilme polimicrobiano |
| Beneficiário: | Fernanda Lourenção Brighenti |
| Modalidade de apoio: | Auxílio à Pesquisa - Regular |
| Processo FAPESP: | 19/26066-4 - Sistemas multiparticulados mucoadesivos para liberação controlada de substâncias naturais |
| Beneficiário: | Luciana Solera Sales |
| Modalidade de apoio: | Bolsas no Brasil - Doutorado |
| Processo FAPESP: | 21/12931-5 - Avaliação anti-inflamatória, antioxidante e antimicrobiana do sistema multiparticulado mucoadesivo contendo substância natural |
| Beneficiário: | Luciana Solera Sales |
| Modalidade de apoio: | Bolsas no Exterior - Estágio de Pesquisa - Doutorado |